FastQCFastQC Report
Wed 8 May 2024
EGAF00008118953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29712
Total Bases8.9 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5761.938610662358643No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5611.8881260096930534No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT5381.810716208939149No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT4161.4001077005923532No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3951.3294291868605277No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3811.2823101777059773No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3811.2823101777059773No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3801.278944534194938No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3721.2520193861066236No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT3511.181340872374798No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3171.0669089929994615No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT3141.0568120624663435No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3141.0568120624663435No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT3031.0197899838449112No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT2960.996230479267636No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2540.8548734518039849No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2530.8515078082929456No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT2510.844776521270867No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT2350.790926225094238No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2330.7841949380721593No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2270.7640010770059236No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2100.7067851373182552No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1970.6630317716747441No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1940.6529348411416263No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1840.6192784060312331No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1800.6058158319870759No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1790.6024501884760366No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1730.5822563274098007No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1620.5452342487883683No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1560.5250403877221325No Hit
CCTACGGGTGGCTGCAGTAGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1530.5149434571890145No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1520.5115778136779752No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1510.5082121701669359No Hit
CCTACGGGTGGCAGCAGTAGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1470.4947495961227787No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1420.47792137856758216No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1400.4711900915455035No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1400.4711900915455035No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1340.4509962304792676No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1340.4509962304792676No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1340.4509962304792676No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1330.44763058696822833No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT1280.4308023694130318No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1260.42407108239095315No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1250.42070543887991385No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1240.4173397953688745No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1240.4173397953688745No Hit
CCTACGGGGGGCTGCAGTAGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1240.4173397953688745No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1230.4139741518578352No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1220.4106085083467959No Hit
CCTACGGGGGGCAGCAGTAGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1210.4072428648357566No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1180.39714593430263867No Hit
CCTACGGGCGGCTGCAGTAGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1110.3735864297253635No Hit
CCTACGGGCGGCAGCAGTAGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1070.36012385568120625No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1030.346661281637049No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT980.3298330640818524No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT950.3197361335487345No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT900.30290791599353795No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT840.28271405492730206No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC820.27598276790522347No Hit
CCTACGGGAGGCAGCAGTAGTCGGCAGCGTCAGATGTGTCATTGTTGGAT800.2692514808831448No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGCGTAGCGAGCTTAT780.26252019386106623No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT740.249057619816909No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC680.22886375875067314No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT630.21203554119547657No Hit
CCTACGGGTGGCTGCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACG610.20530425417339795No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGCGTAGCGAGCTTAT560.1884760366182014No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT560.1884760366182014No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACTCTTAT550.1851103931071621No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT540.18174474959612277No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT530.17837910608508345No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC520.17501346257404415No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT520.17501346257404415No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT520.17501346257404415No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT510.17164781906300483No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGCGTAGCGAGCTTAT490.1649165320409262No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACTCTTAT480.16155088852988692No Hit
CCTACGGGTGGCAGCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACG470.1581852450188476No Hit
CCTACGGGGGGCTGCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACG460.15481960150780827No Hit
CCTACGGGAGGCTGCAGTAGTCGGCAGCGTCAGATGTGTCATTGTTGGAT460.15481960150780827No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGCGTAGCGAGCTTAT460.15481960150780827No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT450.15145395799676897No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACTCTTAT450.15145395799676897No Hit
CCTACGGGGGGCAGCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACG440.14808831448572965No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT430.14472267097469035No Hit
CCTACGGGCGGCTGCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACG420.14135702746365103No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC410.13799138395261173No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC410.13799138395261173No Hit
CCTACGGGTGGCTGCAGCGACAGCGTCAGATGTGTTCACGCCGGATTAGA400.1346257404415724No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT390.13126009693053312No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC370.1245288099084545No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT360.12116316639741519No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACTCTTAT360.12116316639741519No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACTCTTAT360.12116316639741519No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGCGTAGCGAGCTTAT350.11779752288637588No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC350.11779752288637588No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT350.11779752288637588No Hit
CCTACGGGCGGCAGCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACG350.11779752288637588No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGAAACCCTGATG330.11106623586429726No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGCGTAGCGAGCTTAT320.10770059235325795No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGCGTAGCGAGCTTAT320.10770059235325795No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC320.10770059235325795No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT320.10770059235325795No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT310.10433494884221862No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACACTGAT310.10433494884221862No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGAAACCCTGATG310.10433494884221862No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC310.10433494884221862No Hit
CCTACGGGGGGCAGCAGCGACAGCGTCAGATGTGTTCACGCCGGATTAGA300.10096930533117932No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACGATAACTGACG300.10096930533117932No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACTCTTAT300.10096930533117932No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)