FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008118954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29712
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA1440.48465266558966075No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1320.44426494345718903No Hit
GACTACTAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA1140.38368336025848143No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA1140.38368336025848143No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1120.3769520732364028No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1060.3567582121701669No Hit
GACTACCAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA1030.346661281637049No Hit
GACTACCGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA990.3331987075928917No Hit
GACTACCCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA920.30963920301561654No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG890.29954227248249865No Hit
NNNTNNTCGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC840.28271405492730206No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG770.2591545503500269No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG760.2557889068389876No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG750.2524232633279483No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA740.249057619816909No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG720.24232633279483037No Hit
GACTACAAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA700.23559504577275175No Hit
GACTACAGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA690.23222940226171243No Hit
GACTACACGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA690.23222940226171243No Hit
GNNTANTCGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC680.22886375875067314No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG680.22886375875067314No Hit
GACTACTCGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA660.22213247172859452No Hit
GACTACTCGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACTA650.2187668282175552No Hit
GNNTANTAGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC640.2154011847065159No Hit
GNNTANTGGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC630.21203554119547657No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC620.20866989768443725No Hit
NNCTNNTCGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC600.20193861066235863No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA600.20193861066235863No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA580.19520732364028No Hit
GACTACTGGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA570.19184168012924072No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA560.1884760366182014No Hit
GANTANTGGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC560.1884760366182014No Hit
GANTANCCGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC540.18174474959612277No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA540.18174474959612277No Hit
GACTACTAGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA540.18174474959612277No Hit
GANTNNTGGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC520.17501346257404415No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC520.17501346257404415No Hit
GANTNNTCGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC520.17501346257404415No Hit
NANTNNTCGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC510.17164781906300483No Hit
GANTNNCCGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC510.17164781906300483No Hit
NNNTNNCCGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC510.17164781906300483No Hit
GACTACTGGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACTA510.17164781906300483No Hit
NNCTNNTAGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC500.16828217555196553No Hit
GNNTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC500.16828217555196553No Hit
NNNTNNTAGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC500.16828217555196553No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC490.1649165320409262No Hit
GNNTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC490.1649165320409262No Hit
GNNTANCCGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC490.1649165320409262No Hit
NANTNNTGGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC490.1649165320409262No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA460.15481960150780827No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG460.15481960150780827No Hit
GACTACTAGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACTA450.15145395799676897No Hit
NANTANTGGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC450.15145395799676897No Hit
GANTANTCGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC450.15145395799676897No Hit
GACTACCCGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACTA440.14808831448572965No Hit
NANTANTAGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC440.14808831448572965No Hit
GNNTANCAGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC440.14808831448572965No Hit
GACTACCCGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA430.14472267097469035No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG430.14472267097469035No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC430.14472267097469035No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG410.13799138395261173No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG410.13799138395261173No Hit
NNNTNNCAGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC410.13799138395261173No Hit
NANTANCAGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC410.13799138395261173No Hit
NNCTNNTGGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC410.13799138395261173No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA410.13799138395261173No Hit
GANTANTAGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC410.13799138395261173No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG400.1346257404415724No Hit
NNNTNNTCGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC400.1346257404415724No Hit
GNNTANTCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA400.1346257404415724No Hit
GACTACACGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACTA400.1346257404415724No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA390.13126009693053312No Hit
GNNTANCAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA390.13126009693053312No Hit
GNNTANCGGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC390.13126009693053312No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG380.1278944534194938No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC370.1245288099084545No Hit
GANTANACGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC370.1245288099084545No Hit
GANTNNCAGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC370.1245288099084545No Hit
GNNTANCCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA370.1245288099084545No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC370.1245288099084545No Hit
NNNTNNACGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC360.12116316639741519No Hit
NNNTNNTGGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC360.12116316639741519No Hit
NNCTNNCCGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC360.12116316639741519No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG360.12116316639741519No Hit
GACTACAGGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA360.12116316639741519No Hit
GANTNNTAGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC360.12116316639741519No Hit
GNNTANTGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA360.12116316639741519No Hit
GANTANCAGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC360.12116316639741519No Hit
GNNTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC350.11779752288637588No Hit
GNNTANAGGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC350.11779752288637588No Hit
NNNTNNCGGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC350.11779752288637588No Hit
NANTANTCGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC350.11779752288637588No Hit
GANTANCGGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC350.11779752288637588No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG340.11443187937533657No Hit
GACTACAAGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA340.11443187937533657No Hit
NANTNNCAGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC330.11106623586429726No Hit
NANTANCCGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC330.11106623586429726No Hit
GNNTANAAGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC330.11106623586429726No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG320.10770059235325795No Hit
GNNTANTAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA320.10770059235325795No Hit
NNNTNNAAGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC310.10433494884221862No Hit
GANTNNCGGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC310.10433494884221862No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC310.10433494884221862No Hit
NANTANTAGGGTATNTANTNNCGTGAANACNTANNTNACGCTGCCGACGA300.10096930533117932No Hit
GANTANAAGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC300.10096930533117932No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG300.10096930533117932No Hit
GACTACACGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA300.10096930533117932No Hit
NANTNNTAGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC300.10096930533117932No Hit
GACTACCAGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA300.10096930533117932No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)