FastQCFastQC Report
Wed 8 May 2024
EGAF00008118956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60631
Total Bases18.2 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG49708.19712688228794No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG44657.364219623624877No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG35765.897972984117036No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG34315.658821394995959No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG30705.0634164041496925No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG28224.654384720687437No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG26684.400389239827811No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG22243.6680905807260316No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG20043.305239893783708No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2650.4370701456350712No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2620.4321221817222213No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2400.39583711302798896No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2320.3826425425937227No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2210.3645000082466065No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1940.31996833303095773No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1900.31337104781382463No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1880.3100724052052581No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1720.28368326433672547No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1610.26554072998960926No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1580.2605927660767594No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1540.25399548085962625No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1480.24409955303392653No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1420.23420362520822682No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1230.20286652042684436No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1170.19297059260114463No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1170.19297059260114463No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1140.18802262868829478No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1120.18472398607972818No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1070.17647737955831175No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1040.1715294156454619No Hit
GACTACTCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA960.1583348452111956No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA870.143490953472646No Hit
GACTACTGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA850.14019231086407943No Hit
GACTACTAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA800.131945704342663No Hit
GACTACCCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA750.12369909782124655No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA740.12204977651696326No Hit
GACTACCAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA720.11875113390839669No Hit
GACTACCGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA650.10720588477841368No Hit
GACTACTCGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA650.10720588477841368No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA630.1039072421698471No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA630.1039072421698471No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA630.1039072421698471No Hit
GACTACTGGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA620.10225792086556382No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)