FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008118959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97111
Sequences flagged as poor quality0
Sequence length301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC76297.855958645261609No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC61176.298977458784277No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC51505.3032097290729165No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC49035.048861611969808No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC43894.519570388524472No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC32653.3621319932860336No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC27992.8822687440145813No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC22182.2839843066182No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC16191.6671643789066122No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC12721.3098413156079127No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC9841.0132734705646118No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC9700.998856978097229No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC9410.9689942437005076No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9350.9628157469287721No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7150.7362708652984729No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6920.712586627673487No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6670.6868428911245894No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6470.6662479018854712No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6200.6384446664126617No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5920.6096116814778965No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5360.5519457116083657No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5320.547826713760542No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5010.515904480439909No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4440.4572087611084223No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4270.4397030202551719No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4100.4221972794019215No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3940.405721288010627No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3870.39851304177693564No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3830.3943940439291121No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3780.38924529661933255No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3770.3882155471573766No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3520.362471810608479No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3250.3346685751356695No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3150.3243710805161104No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2730.2811216031139624No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2690.27700260526613874No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2660.273913356880271No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2660.273913356880271No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2530.26052661387484427No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2480.2553778665650647No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2380.24508037194550564No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2180.22448538270638754No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2160.22242588378247574No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2100.2162473870107403No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1930.19874164615748985No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1900.19565239777162216No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1830.18844415153793082No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1810.18638465261401901No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1750.18020615584228358No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1720.17711690745641587No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1700.17505740853250404No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1690.17402765907054812No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1670.17196816014663632No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1630.1678491622988127No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1630.1678491622988127No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGTGAAAGCCTGAC1540.15858141714120955No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1500.15446241929338592No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1430.14725417305969457No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1420.14622442359773866No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1370.14107567628795914No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1360.14004592682600325No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1330.1369566784401355No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGTGAAAGCCTGAC1320.13592692897817962No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1320.13592692897817962No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1250.12871868274448828No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1220.12562943435862056No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1200.12356993543470873No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1180.12151043651079693No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1180.12151043651079693No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGTGAAAGCCTGAC1180.12151043651079693No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1150.1184211881249292No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1090.11224269135319377No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1090.11224269135319377No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1080.11121294189123787No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1070.11018319242928196No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1000.10297494619559062No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC980.1009154472716788No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)