FastQCFastQC Report
Wed 8 May 2024
EGAF00008118966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82831
Total Bases24.9 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10941.3207615506271806No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10051.213313855923507No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8501.0261858482935133No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8120.9803093044874504No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7590.9163235986526783No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7230.8728616097837766No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6860.8281923434462943No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6680.8064613490118434No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6000.7243664811483623No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5300.6398570583477201No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5080.6132969540389468No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4910.5927732370730765No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4900.5915659596044959No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4850.5855295722615929No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4710.5686276877014644No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4510.5444821383298524No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4330.5227511438954014No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4260.5143002016153373No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4190.505849259335273No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4100.4949837621180476No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3990.48170370996366096No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3980.48049643249508034No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3770.45514360565488765No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3670.4430708309690817No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3620.4370344436261786No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3610.43582716615759803No Hit
GANTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3300.39840156463159926No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3300.39840156463159926No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3250.3923651772886963No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3210.38753606741437385No Hit
GANTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3120.3766705701971484No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2960.3573541306998588No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2950.35614685323127815No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2920.35252502082553633No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2850.3440740785454721No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2790.3368304137339885No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2770.3344158587968273No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2620.31630669676811823No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2530.3054411995508928No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2530.3054411995508928No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2520.30423392208231215No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2510.3030266446137316No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2480.29940481220798976No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2390.2885393149907643No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2360.28491748258502253No Hit
GANTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2330.2812956501792807No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2290.27646654030495826No Hit
GANTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2260.2728447078992165No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2250.2716374304306359No Hit
GANTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2210.2668083205563135No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2210.2668083205563135No Hit
GANTANCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2170.26197921068199104No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2120.255942823339088No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2040.2462846035904432No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2020.243870048653282No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1930.23300455143605656No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1860.22455360915599232No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1840.22213905421883112No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1830.22093177675025052No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1800.2173099443445087No Hit
GANTANAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1780.21489538940734748No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1750.21127355700160566No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1690.20402989219012205No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1680.20282261472154145No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1620.19557894991005784No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1610.1943716724414772No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1580.19074984003573542No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1580.19074984003573542No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1580.19074984003573542No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1570.1895425625671548No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1530.1847134526928324No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1490.17988434281850998No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1460.17626251041276816No Hit
GANTANAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1400.16901884560128452No Hit
GANTANACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1380.16660429066412333No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1360.16418973572696213No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1350.1629824582583815No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1330.1605679033212203No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG1330.1605679033212203No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1320.15936062585263971No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1280.1545315159783173No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1260.15211696104115607No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1240.14970240610399488No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1190.14366601876109186No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1170.14125146382393067No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1110.13400779901244703No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1070.12917868913812464No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1060.127971411669544No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1050.1267641342009634No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1030.12434957926380219No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1030.12434957926380219No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1020.1231423017952216No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1020.1231423017952216No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG1000.12072774685806038No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1000.12072774685806038No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA980.11831319192089917No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG980.11831319192089917No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG980.11831319192089917No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG960.11589863698373798No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG960.11589863698373798No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG940.11348408204657676No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG940.11348408204657676No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG930.11227680457799616No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG920.11106952710941556No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG900.10865497217225435No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG890.10744769470367374No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG880.10624041723509313No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG870.10503313976651253No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG870.10503313976651253No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA840.10141130736077072No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG830.10020402989219011No Hit
GACTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC830.10020402989219011No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)