FastQCFastQC Report
Wed 8 May 2024
EGAF00008118973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105317
Total Bases31.7 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT102039.687894641890672No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT84928.063275634512946No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT74107.035901136568645No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT67826.439606141458644No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT61695.857553861199996No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT43324.1132960490709No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT36553.4704748521131443No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT33753.204610841554545No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8010.7605609730622787No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5620.5336270497640457No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5530.5250814208532336No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5350.5079901630316093No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4370.4149377593360996No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4060.38550281530996894No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC3930.37315912910546256No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3250.3085921551126599No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3140.29814749755500064No Hit
CCTACGGGTAGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2970.28200575405679995No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2840.2696620678522936No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2810.26681352488202287No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2640.2506717813838222No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2500.23737858085589222No Hit
CCTACGGGTAGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2480.23547955220904504No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2450.23263100923877436No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2200.20889315115318516No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2130.20224655088922017No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT2090.19844849359552588No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT2030.19275140765498447No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2020.19180189333156092No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1990.18895335036129018No Hit
CCTACGGGGAGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1970.18705432171444306No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1950.18515529306759593No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1880.17850869280363094No Hit
CCTACGGGCAGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1850.17566014983336026No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1780.16901354956939527No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1780.16901354956939527No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1700.16141743498200672No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1600.151922291747771No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1570.14907374877750032No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1560.14812423445407674No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1540.14622520580722959No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1450.13767957689641747No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT1420.1348310339261468No Hit
CCTACGGGCAGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1420.1348310339261468No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT1380.1310329766324525No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1380.1310329766324525No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1380.1310329766324525No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT1370.13008346230902892No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1370.13008346230902892No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1340.12723491933875822No Hit
CCTACGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGATG1330.12628540501533467No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1330.12628540501533467No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1320.1253358906919111No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1300.12343686204506395No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1290.12248734772164038No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1290.12248734772164038No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1240.11773977610452252No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1230.11679026178109896No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1220.11584074745767539No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1180.11204269016398113No Hit
CCTACGGGAAGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1170.11109317584055757No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1170.11109317584055757No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1170.11109317584055757No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1160.11014366151713398No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1140.10824463287028685No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1130.10729511854686329No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1120.10634560422343971No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT1090.10349706125316899No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1080.10254754692974542No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT1070.10159803260632186No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1070.10159803260632186No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT1060.1006485182828983No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1060.1006485182828983No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)