FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008118978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112396
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG35413.1504679881846327No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG32532.894231111427453No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG27422.439588597458984No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG26712.3764190896473183No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG25462.265205167443681No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG24362.167336915904481No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG22311.9849460834905157No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG20921.861276202000071No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG20451.8194597672515036No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG20411.8159009217409872No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG19151.703797288159721No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG17341.5427595288088545No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG16911.5045019395708032No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG15721.3986262856329406No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG15631.3906188832342787No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG15571.3852806149685042No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13291.18242642086907No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG11731.0436314459589309No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11621.0338446208050107No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG10100.8986084914053881No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG8210.7304530410334887No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7770.6913057404178085No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7510.6681732445994519No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6840.6085625822983024No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6830.6076728709206733No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6610.5880992206128333No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6390.5685255703049931No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6290.5596284565287021No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6210.5525107655076693No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6150.5471724972418948No Hit
GANTANACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5500.4893412576960034No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5080.45197337983558133No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5030.44752482294743584No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4960.4412968433040322No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4820.4288408840172248No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4640.41282607921990105No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4380.38969358340154453No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4280.3807964696252536No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4160.3701199330937044No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3720.3309726324780241No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3630.3229652300793623No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3340.29716360012811843No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3270.2909356204847148No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3120.2775899498202783No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2950.26246485640058365No Hit
GACTANTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2900.25801629951243815No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2810.2500088971137763No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2750.2446706288480017No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2690.23933236058222715No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2530.22509697854016156No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2480.2206484216520161No Hit
GACTANCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2460.2188689988967579No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2450.2179792875191288No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2440.2170895761414997No Hit
GACTANTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2390.2126410192533542No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2340.20819246236520872No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2330.20730275098757964No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2290.20374390547706323No Hit
GACTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2270.20196448272180503No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2220.19751592583365957No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2100.1868393893021104No Hit
GACTANTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2090.1859496779244813No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2060.183280543791594No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2040.1815011210363358No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2040.1815011210363358No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2020.17972169828107762No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2010.17883198690344854No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1970.17527314139293212No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1970.17527314139293212No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1900.16904516174952844No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1890.16815545037189936No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1860.16548631623901205No Hit
GACTANCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1840.16370689348375386No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1800.16014804797323748No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1800.16014804797323748No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1750.155699491085092No Hit
GACTANCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1750.155699491085092No Hit
GACTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1670.14858180006405922No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1670.14858180006405922No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1660.14769208868643013No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1660.14769208868643013No Hit
GACTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1610.14324353179828464No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1600.14235382042065553No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1590.14146410904302645No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1580.14057439766539734No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1550.13790526353251004No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1540.13701555215488095No Hit
GACTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1510.13434641802199365No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1510.13434641802199365No Hit
GACTANAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1510.13434641802199365No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1490.1325669952667355No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1480.13167728388910638No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1480.13167728388910638No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1470.13078757251147727No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1460.12989786113384819No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1450.12900814975621908No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1450.12900814975621908No Hit
GACTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1440.12811843837859No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1440.12811843837859No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1430.12722872700096088No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1420.12633901562333177No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1410.1254493042457027No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1390.1236698814904445No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1390.1236698814904445No Hit
GACTANAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1360.12100074735755721No Hit
GACTANACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1340.119221324602299No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1290.11477276771415353No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1270.11299334495889533No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1250.11121392220363713No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1230.10943449944837895No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1230.10943449944837895No Hit
GANTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1220.10854478807074985No Hit
GANTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1210.10765507669312074No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1200.10676536531549165No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1200.10676536531549165No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1200.10676536531549165No Hit
GACTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1190.10587565393786257No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1190.10587565393786257No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1180.10498594256023346No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1170.10409623118260436No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1150.10231680842734618No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1150.10231680842734618No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1130.10053738567208798No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)