FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008118996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80430
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8501.0568195946786025No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7320.9101081685938083No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6790.8442123585726719No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6430.7994529404451075No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6210.7720999627004849No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5920.7360437647643914No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5350.6651746860624145No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4890.607982096232749No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4860.6042521447221185No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4650.5781424841477061No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4630.5756558498072858No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4630.5756558498072858No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4050.5035434539350988No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3950.49111028223299763No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3810.473703841850056No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3750.4662439388287952No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3740.4650006216585851No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3610.4488374984458535No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3580.4451075469352232No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3570.44386422976501305No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3410.4239711550416511No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3410.4239711550416511No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3370.41899788636081065No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3250.4040780803182892No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3120.3879149571055576No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2940.36553524804177545No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2760.3431555389779933No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2740.34066890463757304No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2640.32823573293547187No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2630.3269924157652617No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2600.32326246425463134No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2470.3070993410418998No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2400.29839612085042894No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2310.28720626631853785No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2180.2710431431058063No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2170.26979982593559615No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2170.26979982593559615No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2140.26606987442496577No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2100.26109660574412535No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2060.2561233370632849No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2010.24990675121223427No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1930.23996021385055327No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1880.23374362799950268No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1810.22504040780803183No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1790.2225537734676116No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1760.21882382195698125No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1720.21385055327614072No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1700.21136391893572049No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1680.20887728459530025No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1670.20763396742509016No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1610.20017406440382943No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1600.19893074723361928No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1570.19520079572298893No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1410.175307720999627No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1390.17282108665920676No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1380.17157776948899664No Hit
NNNTNNTGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG1330.16536118363794605No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1290.16038791495710555No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1290.16038791495710555No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1290.16038791495710555No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG1270.15790128061668532No Hit
NANTNNTCGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG1260.1566579634464752No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1250.15541464627626508No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1240.15417132910605494No Hit
GNNTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1230.15292801193584482No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1230.15292801193584482No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1230.15292801193584482No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1220.1516846947656347No Hit
NNCTNNTCGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG1210.15044137759542459No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1210.15044137759542459No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1190.14795474325500435No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1180.14671142608479423No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1160.144224791744374No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1150.14298147457416388No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1130.14049484023374362No Hit
NNNTNNTGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG1120.1392515230635335No Hit
GNNTANTGGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG1120.1392515230635335No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1110.13800820589332338No Hit
NNNTNNCGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG1090.13552157155290315No Hit
NNNTNNTCGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG1090.13552157155290315No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1090.13552157155290315No Hit
GANTNNTGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG1080.134278254382693No Hit
GANTANTGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG1080.134278254382693No Hit
GNNTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1070.13303493721248288No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1070.13303493721248288No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1060.1317916200422728No Hit
NANTANTGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG1050.13054830287206268No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1050.13054830287206268No Hit
NNNTNNTCGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG1040.12930498570185256No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1040.12930498570185256No Hit
NNCTNNTGGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG1040.12930498570185256No Hit
GANTNNTCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG1040.12930498570185256No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1030.12806166853164244No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1030.12806166853164244No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1030.12806166853164244No Hit
GNNTANTCGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG1030.12806166853164244No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1030.12806166853164244No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1020.1268183513614323No Hit
GNNTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1010.12557503419122218No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1000.12433171702101206No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG990.12308839985080194No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG990.12308839985080194No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG970.12060176551038168No Hit
GANTANTCGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG970.12060176551038168No Hit
GACTACTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG970.12060176551038168No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG960.11935844834017158No Hit
NANTNNTAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG960.11935844834017158No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG960.11935844834017158No Hit
NANTNNCGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG950.11811513116996146No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG950.11811513116996146No Hit
GNNTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG940.11687181399975134No Hit
GNNTANTAGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG930.11562849682954122No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG920.11438517965933111No Hit
NNCTNNTAGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG920.11438517965933111No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG920.11438517965933111No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG900.11189854531891084No Hit
GNNTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG900.11189854531891084No Hit
GANTNNCGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG890.11065522814870073No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG880.10941191097849062No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG880.10941191097849062No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG880.10941191097849062No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG870.1081685938082805No Hit
NNNTNNTAGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG870.1081685938082805No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG860.10692527663807036No Hit
NNCTNNCGGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG860.10692527663807036No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG860.10692527663807036No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG860.10692527663807036No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG850.10568195946786024No Hit
NNNTNNCCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG850.10568195946786024No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG850.10568195946786024No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG850.10568195946786024No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG850.10568195946786024No Hit
GANTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG840.10443864229765012No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG840.10443864229765012No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG840.10443864229765012No Hit
GANTANTAGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG830.10319532512744001No Hit
NNNTNNTAGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG820.10195200795722989No Hit
NNNTNNCGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG820.10195200795722989No Hit
GANTNNTAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG820.10195200795722989No Hit
NNNTNNCCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG810.10070869078701977No Hit
GANTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG810.10070869078701977No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG810.10070869078701977No Hit
GANTANCGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG810.10070869078701977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)