FastQCFastQC Report
Wed 8 May 2024
EGAF00008119002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92423
Total Bases27.8 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG22772.4636724624822826No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG20322.1985869318243294No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG16271.7603843199203661No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG15091.6327104725014334No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13231.4314618655529467No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12531.3557231425078173No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12431.3449033249299418No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10521.138244809192517No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9791.0592601408740248No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6540.7076160695930667No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5660.612401674907761No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5220.5647944775651083No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5000.5409908788937818No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4780.5171872802224554No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4500.4868917910044037No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4180.45226837475520165No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3820.41331703147484933No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3760.40682514092812394No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3570.38626748753016027No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3560.3851855057723727No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3120.33757830842971986No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3100.33541434491414474No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3040.32892245436741935No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG3030.3278404726096318No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3020.3267584908518442No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2990.3235125455784815No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2960.32026660030511883No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG2810.30403687393830536No Hit
GNNTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2580.27915129350919143No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2570.27806931175140387No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG2530.2737413847202536No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG2510.2715774212046785No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2440.26400354890016553No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG2420.2618395853845904No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG2390.2585936401112277No Hit
GANTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2380.25751165835344014No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG2370.2564296765956526No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG2360.255347694837865No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG2270.245609859017777No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG2270.245609859017777No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG2210.2391179684710516No Hit
GANTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2190.23695400495547647No Hit
GNNTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2170.23479004143990134No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG2160.23370805968211378No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2150.2326260779243262No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2120.22938013265096352No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG2110.22829815089317596No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG2090.22613418737760083No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG2070.2239702238620257No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG2060.22288824210423813No Hit
GANTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2030.21964229683087544No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG2010.2174783333153003No Hit
NNNTNNCGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG2000.21639635155751274No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1990.21531436979972518No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1960.2120684245263625No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1910.20665851573742466No Hit
GANTANCGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1910.20665851573742466No Hit
NANTANTAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1880.20341257046406197No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1870.2023305887062744No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1830.19800266167512415No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1790.19367473464397392No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1780.19259275288618635No Hit
NNCTNNCGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1770.1915107711283988No Hit
NNNTNNTAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1770.1915107711283988No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1760.19042878937061122No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1740.1882648258550361No Hit
GANTANTAGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1730.18718284409724853No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1690.18285491706609827No Hit
NNCTNNTAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1660.17960897179273558No Hit
GNNTANAGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1650.178526990034948No Hit
GANTNNTAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1640.17744500827716045No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1630.17636302651937288No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1630.17636302651937288No Hit
GNNTANCCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1610.17419906300379775No Hit
NNNTNNCCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1610.17419906300379775No Hit
NANTNNAGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1590.17203509948822263No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1570.1698711359726475No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1520.16446122718370967No Hit
NANTNNCAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1510.1633792454259221No Hit
GANTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1510.1633792454259221No Hit
NANTANCGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1500.16229726366813454No Hit
GNNTANTAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1470.15905131839477185No Hit
NNCTNNCCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1470.15905131839477185No Hit
GANTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1460.1579693366369843No Hit
NANTNNACGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1440.15580537312140916No Hit
GANTANCAGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1420.15364140960583403No Hit
GNNTANCAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1400.1514774460902589No Hit
NNNTNNCAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1400.1514774460902589No Hit
GANTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1400.1514774460902589No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1400.1514774460902589No Hit
NNNTNNCAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1380.1493134825746838No Hit
NNCTNNAGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1380.1493134825746838No Hit
NANTANACGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1360.14714951905910867No Hit
GANTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1350.1460675373013211No Hit
GANTNNCAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1340.14498555554353354No Hit
NNNTNNAGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1340.14498555554353354No Hit
NNCTNNCAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1340.14498555554353354No Hit
NNNTNNCCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1330.14390357378574598No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1300.14065762851238328No Hit
GANTANAGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1290.13957564675459572No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1290.13957564675459572No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1270.1374116832390206No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1260.13632970148123302No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1250.13524771972344546No Hit
GANTNNCCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1250.13524771972344546No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1240.1341657379656579No Hit
NNNTNNAGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1230.13308375620787033No Hit
GANTANCCGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1210.1309197926922952No Hit
NANTANAGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1170.12659186566114494No Hit
NNCTNNACGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1170.12659186566114494No Hit
GANTNNAGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1170.12659186566114494No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1160.12550988390335738No Hit
NNNTNNAAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1160.12550988390335738No Hit
NANTANCCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1160.12550988390335738No Hit
NANTNNAAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1160.12550988390335738No Hit
NANTANCAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1120.12118195687220715No Hit
GNNTANACGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1110.12009997511441958No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1090.11793601159884445No Hit
GANTNNAAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1080.11685402984105689No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1050.1136080845676942No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1050.1136080845676942No Hit
GANTNNACGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1040.11252610280990663No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG1030.11144412105211907No Hit
GNNTANAAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1030.11144412105211907No Hit
NNNTNNACGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1030.11144412105211907No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1030.11144412105211907No Hit
GANTANACGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1020.1103621392943315No Hit
GANTANAAGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1010.10928015753654394No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1010.10928015753654394No Hit
GANTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1000.10819817577875637No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1000.10819817577875637No Hit
NNNTNNACGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG990.10711619402096881No Hit
GACTACTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG980.10603421226318124No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG970.10495223050539368No Hit
GACTACTCGGGTATCTAATCCGGAGACCTTCTTCCTCAACGCGGCGTCGC970.10495223050539368No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG950.10278826698981855No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG940.10170628523203099No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC940.10170628523203099No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC940.10170628523203099No Hit
NNNTNNAAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG930.10062430347424342No Hit
GACTACCGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG930.10062430347424342No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)