FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008119052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91409
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC20732.267829207189664No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC20402.2317277292170354No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG15701.7175551641523263No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC15381.6825476703606865No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG14621.599404872605542No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC14351.569867299718846No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC14051.5370477742891837No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12881.4090516251135008No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11831.2941832861096827No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11211.2263562668883807No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10571.1563412793051013No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10391.1366495640473038No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10031.097266133531709No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9020.9867737312518461No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8700.9517662374602063No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8590.9397324114693302No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7050.7712588475970638No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6640.7264054961765253No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA6490.7099957334616941No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA6280.6870220656609306No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4720.5163605334266866No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4430.4846349921780131No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4380.47916507127306934No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3800.41571398877572235No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC3390.3708606373551838No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC3330.36429673226925136No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3300.36101477972628515No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA3250.3555448588213414No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3230.35335689045936397No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2900.31725541248673544No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2850.31178549158179175No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2840.310691507400803No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2730.29865768140992677No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2680.2931877605049831No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2430.2658381559802645No Hit
GACTACTGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA2430.2658381559802645No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2400.2625562034372983No Hit
GACTACTCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA2350.25708628253235455No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2320.25380432998938834No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2280.2494283932654334No Hit
GACTACCAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2220.2428644881795009No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2130.23301863055060226No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2110.23083066218862475No Hit
GACTACCGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2100.22973667800763603No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1990.21770285201675985No Hit
GACTACTAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1970.21551488365478236No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1950.2133269152928049No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1940.21223293111181613No Hit
GACTACCCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1940.21223293111181613No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1890.2067630102068724No Hit
GACTACACGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1880.20566902602588366No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1800.1969171525779737No Hit
GACTACAGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1690.18488332658709755No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1670.18269535822512006No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1640.17941340568215383No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1580.1728495005962214No Hit
GACTACCGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1550.16956754805325513No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1530.16737957969127767No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1510.1651916113293002No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1510.1651916113293002No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1480.16190965878633395No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1460.15972169042435647No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1440.15753372206237898No Hit
GACTACAAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1420.1553457537004015No Hit
GACTACCAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1340.14659388025249154No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1320.14440591189051408No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCCTCAG1310.14331192770952533No Hit
GACTACACGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1270.13893599098557036No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1220.13346607008062664No Hit
GACTACAAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1220.13346607008062664No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1200.13127810171864915No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCCTCAG1180.12909013335667166No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1170.12799614917568292No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1170.12799614917568292No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1150.12580818081370543No Hit
GACTACTGGGGTATCTAATCCGAAGACCTCCTTCCTCCACGCGGCGTCGC1140.1247141966327167No Hit
GACTACAGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1110.12143224408975045No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA1110.12143224408975045No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1080.11815029154678422No Hit
GACTACTCGGGTATCTAATCCGAAGACCTCCTTCCTCCACGCGGCGTCGC1070.11705630736579549No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA1070.11705630736579549No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1020.11158638646085177No Hit
GACTACTAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA1010.11049240227986304No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA1010.11049240227986304No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA990.10830443391788554No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA980.1072104497368968No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC970.10611646555590806No Hit
GACTACTGGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA940.10283451301294183No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA940.10283451301294183No Hit
GACTACTAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA940.10283451301294183No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCCTCAG940.10283451301294183No Hit
GACTACTGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA930.10174052883195309No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA920.10064654465096436No Hit
GACTACTCGGGTATCTAATCCTGATATCATACATCTGACGCTGCCGACGA920.10064654465096436No Hit
GACTACTGGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA920.10064654465096436No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCCTCAG920.10064654465096436No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)