FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008119061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187564
Sequences flagged as poor quality0
Sequence length301
%GC57

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT105875.644473353095477No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT90114.8042268239107715No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT75654.033289970356785No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT75564.0284916081977356No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT72773.879742381267194No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT62983.357787208632787No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT62043.3076709816382674No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT54492.9051417116290974No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT53842.8704868738137383No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT45452.423172890320104No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT42022.2403019769252097No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGGACAATGGGGGAAACCCTGAT41302.201915079652812No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT37702.0099805932908237No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGGACAATGGGGGAAACCCTGAT34261.8265765285449234No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT30811.6426393124480176No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGGACAATGGGGGAAACCCTGAT30291.6149154421957306No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT29861.5919899341024932No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGGACAATGGGGGAAACCCTGAT28381.5130835341536757No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT26971.4379091936618968No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGGACAATGGGGGAAACCCTGAT23291.2417094964918642No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT21881.1665351560000854No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGGACAATGGGGGAAACCCTGAT17220.9180866264315114No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT15760.840246529184705No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGGACAATGGGGGAAACCCTGAT15730.8386470751316883No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT12860.6856326373931031No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGGACAATGGGGGAAACCCTGAT12440.6632402806508712No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT11470.6115245996033354No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT11190.5965963617751807No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT9270.4942313023821202No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7220.38493527542598793No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5900.3145592970932588No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5700.3038962700731484No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT5480.29216694035102686No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4870.2596447079396899No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4500.23991810795248558No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC4480.2388518052504745No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT4440.2367191998464524No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT4130.22019150796528117No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3980.21219423770019835No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3930.20952848094517074No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3900.20792902689215415No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT3870.20632957283913755No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3780.20153121068008784No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC3680.19619969717003263No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3500.1866029728519332No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3480.18553667014992214No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3280.17487364312981168No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3230.17220788637478407No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3220.17167473502377853No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3200.1706084323217675No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3160.1684758269177454No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3040.16207801070567912No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT2980.158879102599646No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC2950.1572796485466294No Hit
CCTATGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2930.15621334584461835No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC2860.1524812863875797No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGCGGAAGCCTGAC2850.1519481350365742No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2820.1503486809835576No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2700.14395086477149133No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC2650.1412851080164637No Hit
CCTATGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2580.13755304855942507No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2540.13542044315540297No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2490.13275468640037533No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2410.12848947559233115No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGGACAATGGGCGAAAGCCTGAT2390.1274231728903201No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGCGGAAGCCTGAC2220.1183595999232262No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGGACAATGGGCGAAAGCCTGAT2200.11729329722121515No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2180.1162269945192041No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC2180.1162269945192041No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT2160.11516069181719307No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2130.1135612377641765No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGGACAATGGGCGAAAGCCTGAT2090.11142863236015439No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1990.10609711885009918No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1990.10609711885009918No Hit
CCTATGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1960.10449766479708261No Hit
CCTATGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1930.10289821074406602No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1920.10236505939306051No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1920.10236505939306051No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)