FastQCFastQC Report
Wed 8 May 2024
EGAF00008119062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187564
Total Bases56.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG58363.1114712844682346No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG53292.8411635495084346No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG44642.3799876308886567No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG44072.3495980038813418No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG42742.2786888741976075No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG40622.1656607877844363No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG35411.8878889339105587No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG34121.819112409630846No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG33601.791388539378559No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG33031.760998912371244No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG32611.738606555629012No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG31121.659167004329189No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG31021.6538354908191337No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG27851.4848265125503828No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG26521.4139173828666483No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG25741.3723315774882174No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG25401.3542044315540296No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG24881.3264805613017423No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG24851.3248811072487259No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG24761.3200827450896762No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG20971.1180183830585828No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG19861.0588385830969695No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG19671.0487087074278647No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG18951.010321810155467No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG18520.9873963020622295No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG16330.8706361561920197No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG14970.7981275724552687No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG14830.7906634535411912No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG12960.6909641509031583No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11140.5939306050201532No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG10290.5486127401846836No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG9880.5267535347934572No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9230.49209869697809816No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG9190.489966091574076No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG8490.4526454970036894No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG8450.45051289159966734No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8370.44624768079162314No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG8260.44038301593056234No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7660.4083939348702309No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7480.3987972105521315No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG7430.3961314537971039No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7020.3742722484058775No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG6870.3662749781407946No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6850.36520867543878355No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6450.3438826213985626No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6310.3364185024844853No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6200.33055383762342455No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5930.3161587511462754No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5740.3060288754771705No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5640.3006973619671152No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG5080.27084088631080594No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5000.26657567550276173No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4910.261777313343712No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4850.2585784052376789No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG4640.2473822268665629No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4590.24471647011153527No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG4350.23192083768740268No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG4240.22605617282634194No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4240.22605617282634194No Hit
GACTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4240.22605617282634194No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG4030.21485999445522594No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3950.21059478364718176No Hit
GACTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3920.20899532959416522No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3610.19246763771299397No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG3430.18287091339489456No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3410.1818046106928835No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3390.18073830799087245No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG3300.17593994583182274No Hit
GACTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3280.17487364312981168No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3240.17274103772578958No Hit
GACTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3130.16687637286472884No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3070.1636774647586957No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3020.16101170800366807No Hit
GACTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2980.158879102599646No Hit
GACTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2880.15354758908959076No Hit
GACTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2770.14768292422853No Hit
GACTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2700.14395086477149133No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2690.1434177134204858No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2660.14181825936746922No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2560.13648674585741402No Hit
GACTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2520.13435414045339192No Hit
GACTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2480.13222153504936981No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2470.1316883836983643No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2410.12848947559233115No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2410.12848947559233115No Hit
GACTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2370.12635687018830905No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2310.1231579620822759No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2230.11889275127423174No Hit
GACTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2200.11729329722121515No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2160.11516069181719307No Hit
GACTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2150.11462754046618753No Hit
GACTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2110.11249493506216544No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2100.11196178371115993No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2080.11089548100914888No Hit
GACTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2050.10929602695613232No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2040.10876287560512678No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2030.10822972425412127No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2020.10769657290311573No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2010.10716342155211021No Hit
GACTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1960.10449766479708261No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1960.10449766479708261No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1940.10343136209507156No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1940.10343136209507156No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1940.10343136209507156No Hit
GACTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1930.10289821074406602No Hit
GACTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1920.10236505939306051No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)