FastQCFastQC Report
Tue 7 May 2024
EGAF00008119078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21720
Total Bases6.5 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG10574.866482504604051No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG9624.429097605893186No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG9224.244935543278085No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7283.3517495395948433No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG7283.3517495395948433No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG7233.3287292817679557No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6923.1860036832412524No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6813.1353591160220997No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG6462.9742173112338857No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5882.707182320441989No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5732.638121546961326No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5392.4815837937384897No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4932.2697974217311234No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4902.255985267034991No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4031.8554327808471456No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3761.7311233885819521No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3571.643646408839779No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3531.6252302025782688No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG460.2117863720073665No Hit
GACTACTGGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGTGCATGAG460.2117863720073665No Hit
GACTACTGGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGTGCATGAG440.20257826887661143No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG420.19337016574585636No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG380.17495395948434622No Hit
GACTACCGGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGTGCATGAG370.1703499079189687No Hit
GACTACTGGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG360.16574585635359115No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG350.16114180478821363No Hit
GACTACTCGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG340.15653775322283608No Hit
GACTACTGGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGCACCTCAG310.1427255985267035No Hit
GACTACCCGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGTGCATGAG300.13812154696132595No Hit
GACTACCCGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG300.13812154696132595No Hit
GACTACTAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGTGCATGAG300.13812154696132595No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG290.13351749539594843No Hit
GACTACAAGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGTGCATGAG280.1289134438305709No Hit
GACTACTCGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGCACCTCAG280.1289134438305709No Hit
GACTACTAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG280.1289134438305709No Hit
GACTACTCGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGTGCATGAG280.1289134438305709No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG280.1289134438305709No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATCAG280.1289134438305709No Hit
GACTACCAGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGTGCATGAG270.12430939226519336No Hit
GACTACTCGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGTGCATGAG270.12430939226519336No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG270.12430939226519336No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATCAG270.12430939226519336No Hit
GACTACCCGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGCACCTCAG260.11970534069981582No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATCAG260.11970534069981582No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG260.11970534069981582No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG260.11970534069981582No Hit
GACTACCCGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGTGCATGAG260.11970534069981582No Hit
GACTACCGGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGTGCATGAG260.11970534069981582No Hit
GACTACAGGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGTGCATGAG250.11510128913443832No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG250.11510128913443832No Hit
GACTACCGGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG240.11049723756906078No Hit
GACTACACGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGTGCATGAG230.10589318600368325No Hit
GACTACCGGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGCACCTCAG230.10589318600368325No Hit
GACTACAGGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGCACCTCAG230.10589318600368325No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG230.10589318600368325No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATGAG230.10589318600368325No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA220.10128913443830571No Hit
GACTACCAGGGTATCTAATCNTGTTTGNTCCCCACGNTTTCGCACCTCAG220.10128913443830571No Hit
GACTACCAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG220.10128913443830571No Hit
GACTACCAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGTGCATGAG220.10128913443830571No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)