FastQCFastQC Report
Wed 8 May 2024
EGAF00008119094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64775
Total Bases19.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG9091.4033191817830952No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG9041.395600154380548No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG7071.0914704747201853No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG6701.0343496719413354No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG6410.9895793130065611No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG5310.819760710150521No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG4940.7626399073716711No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG4620.7132381319953686No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG3690.5696642223079892No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2630.40602084137398686No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2580.3983018139714396No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2570.3967580084909301No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2370.365881898880741No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2220.3427248166730992No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2160.33346198379004244No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2080.3211115399459668No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1970.3041296796603628No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1910.29486684677730607No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1820.28097259745272096No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1740.2686221536086453No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1680.25935932072558854No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1640.25318409880355075No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG1620.2500964878425318No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1590.2454650714010035No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG1520.2346584330374373No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1510.23311462755692786No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG1500.23157082207641835No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1480.22848321111539946No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG1480.22848321111539946No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1470.22693940563489No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG1470.22693940563489No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG1440.22230798919336164No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG1400.21613276727132383No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1400.21613276727132383No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG1390.21458896179081438No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1370.21150135082979543No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG1370.21150135082979543No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1360.20995754534928598No Hit
GNNTANACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1360.20995754534928598No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1330.20532612890775764No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG1330.20532612890775764No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG1310.2022385179467387No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG1280.19760710150521035No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG1280.19760710150521035No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG1270.1960632960247009No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG1250.19297568506368198No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG1240.1914318795831725No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1230.18988807410266306No Hit
GANTANTAGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG1230.18988807410266306No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG1230.18988807410266306No Hit
GNNTANAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1180.1821690467001158No Hit
NNCTNNTAGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG1170.18062524121960633No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1160.17908143573909688No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1160.17908143573909688No Hit
NNNTNNTAGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG1160.17908143573909688No Hit
GNNTANCCGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG1140.17599382477807796No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1140.17599382477807796No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG1140.17599382477807796No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG1120.17290621381705906No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG1110.1713624083365496No Hit
GANTANCGGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG1080.16673099189502122No Hit
GANTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1080.16673099189502122No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG1070.16518718641451177No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG1070.16518718641451177No Hit
NANTNNAGGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG1060.16364338093400232No Hit
GANTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1040.1605557699729834No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1040.1605557699729834No Hit
NANTNNCAGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG1000.1543805480509456No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1000.1543805480509456No Hit
NNNTNNCGGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG1000.1543805480509456No Hit
NNCTNNCGGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG960.14820532612890774No Hit
GNNTANTAGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG950.1466615206483983No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG950.1466615206483983No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG950.1466615206483983No Hit
GANTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG940.14511771516788885No Hit
NANTANTAGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG930.14357390968737938No Hit
GANTANCCGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG930.14357390968737938No Hit
NNNTNNAGGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG920.14203010420686993No Hit
NNNTNNCCGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG910.14048629872636048No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG900.138942493245851No Hit
NNNTNNCCGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG900.138942493245851No Hit
GANTNNTAGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG900.138942493245851No Hit
NANTANCCGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG890.13739868776534156No Hit
GANTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG890.13739868776534156No Hit
GANTNNCCGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG890.13739868776534156No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC890.13739868776534156No Hit
NNCTNNCCGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG880.1358548822848321No Hit
NANTNNACGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG880.1358548822848321No Hit
NNCTNNCAGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG870.13431107680432264No Hit
GANTNNCAGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG870.13431107680432264No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG870.13431107680432264No Hit
GNNTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC850.13122346584330374No Hit
GNNTANCAGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG830.12813585488228482No Hit
GNNTANCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG820.12659204940177538No Hit
NANTANCGGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG820.12659204940177538No Hit
NNNTNNCAGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG820.12659204940177538No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC820.12659204940177538No Hit
GANTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG820.12659204940177538No Hit
NNCTNNAGGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG810.1250482439212659No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC810.1250482439212659No Hit
GNNTANAGGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG800.12350443844075647No Hit
NNNTNNCAGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG790.121960632960247No Hit
NNNTNNAGGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG780.12041682747973755No Hit
NNCTNNACGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG780.12041682747973755No Hit
GANTANACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG770.1188730219992281No Hit
GANTANCAGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG760.11732921651871865No Hit
GANTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG760.11732921651871865No Hit
GNNTANACGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG750.11578541103820918No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG740.11424160555769973No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG730.11269780007719028No Hit
GNNTANTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG730.11269780007719028No Hit
GANTANAGGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG720.11115399459668082No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG710.10961018911617136No Hit
NANTNNAAGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG710.10961018911617136No Hit
GANTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG700.10806638363566191No Hit
NNNTNNACGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG700.10806638363566191No Hit
NANTANCAGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG690.10652257815515247No Hit
GNNTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC670.10343496719413355No Hit
NNCTNNAAGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG670.10343496719413355No Hit
GANTNNAGGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG650.10034735623311462No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)