FastQCFastQC Report
Wed 8 May 2024
EGAF00008119098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74527
Total Bases22.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6490.8708253384679377No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5930.7956847853797953No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4450.5970990379325614No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4080.5474526010707529No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4070.5461108054798932No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4030.5407436231164545No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3440.4615776832557328No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3220.43205818025681964No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3130.4199820199390825No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2930.3931461081218887No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2730.3663101963046949No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2560.34349967126008024No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2110.28311886967139427No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2070.2777516873079555No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2050.2750680961262361No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2010.26970091376279737No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1970.2643337313993586No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1740.2334724328095858No Hit
GNNTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1690.22676345485528734No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1620.21737088571926952No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1600.21468729453755017No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1560.2093201121741114No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1530.20529472540153235No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1400.18785138272035637No Hit
GNNTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1390.1865095871294967No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1370.18382599594777732No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1350.18114240476605792No Hit
GNNTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1320.17711701799347887No Hit
GNNTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1290.17309163122089982No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1160.15564828853972387No Hit
NNNTNNTGGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC1120.1502811061762851No Hit
GNNTANAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1050.1408885370402673No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1050.1408885370402673No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1020.13686315026768822No Hit
NANTNNTGGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC1020.13686315026768822No Hit
GNNTANACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1010.13552135467682852No Hit
GNNTANCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC970.13015417231338977No Hit
GNNTANTCGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC970.13015417231338977No Hit
NANTNNTCGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC930.12478698994995102No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG930.12478698994995102No Hit
GNNTANTGGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC910.12210339876823165No Hit
GANTANTGGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC910.12210339876823165No Hit
NNNTNNTCGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC900.12076160317737196No Hit
NNCTNNTGGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC900.12076160317737196No Hit
GANTANTCGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC890.11941980758651226No Hit
NANTANTCGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC880.11807801199565258No Hit
NNCTNNTCGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC870.1167362164047929No Hit
NNNTNNTCGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC870.1167362164047929No Hit
GNNTANAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC870.1167362164047929No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG860.1153944208139332No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA830.11136903404135413No Hit
NNNTNNTGGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC830.11136903404135413No Hit
GANTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC820.11002723845049446No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG820.11002723845049446No Hit
GANTNNTGGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC820.11002723845049446No Hit
GANTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC820.11002723845049446No Hit
NNNTNNCCGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC770.10331826049619602No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG770.10331826049619602No Hit
GANTNNCCGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC770.10331826049619602No Hit
NANTNNCCGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC760.10197646490533632No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG750.10063466931447664No Hit
NANTANTGGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC750.10063466931447664No Hit
NNNTNNTAGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC750.10063466931447664No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)