FastQCFastQC Report
Wed 8 May 2024
EGAF00008119116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79898
Total Bases24 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG41745.224160805026409No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG38624.833662920223285No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG35064.388094820896643No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG33264.162807579664072No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG32614.081453853663421No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG30483.814863951538211No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG28803.6045958597211443No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG27403.429372449873589No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG26213.2804325515031665No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7970.9975218403464418No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7890.9875090740694386No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6660.8335627925605147No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6560.8210468347142607No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6530.8172920473603845No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6520.8160404515757591No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6480.8110340684372574No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG6360.7960149190217527No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG5930.7421963002828607No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5650.7071516183133495No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5600.7008936393902225No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5550.6946356604670956No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5400.6758617236977146No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG5310.6645973616360861No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG5180.6483266164359559No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5080.6358106585897019No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5070.6345590628050765No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG5030.6295526796665749No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4760.5957595934816892No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4700.5882500187739368No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG4690.5869984229893114No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG4570.5719792735738066No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG4520.5657212946506797No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4500.5632181030814288No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4360.5456957620966733No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4130.516909059050289No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4010.5018899096347843No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG3970.49688352649628276No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3890.48687076021927955No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3630.4543292698190192No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG3500.43805852461888906No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG3460.43305214148038745No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3450.4318005456957621No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3390.4242909709880097No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3290.41177501314175574No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3280.4105234173571303No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3080.38549150166462237No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3040.3804851185261208No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3020.37798192695687No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3010.37673033117224464No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2930.36671756489524143No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2930.36671756489524143No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2920.365465969110616No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2910.36421437332599066No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2810.3516984154797367No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2770.3466920323412351No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2750.3441888407719843No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2710.3391824576334827No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2660.3329244787103557No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2610.3266664997872287No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2580.32291171243335254No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2570.3216601166487271No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2570.3216601166487271No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2540.31790532929485094No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2510.31415054194097475No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2420.3028861798793462No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2400.3003829883100954No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2330.2916218178177176No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2310.2891186262484668No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2300.28786703046384143No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2290.286615434679216No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2280.2853638388945906No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2090.261583518986708No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2050.25657713584820646No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2050.25657713584820646No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1990.24906756114045406No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1970.24656436957120326No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG1870.2340484117249493No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1870.2340484117249493No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1840.23029362437107312No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1820.22779043280182232No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1750.21902926230944453No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG1730.21652607074019373No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG1700.21277128338631754No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1660.20776490024781596No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG1660.20776490024781596No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1660.20776490024781596No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1630.20401011289393978No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1530.1914941550476858No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1490.1864877719091842No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1400.17522340984755563No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1310.16395904778592707No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1310.16395904778592707No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1300.16270745200130166No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1290.16145585621667627No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1240.15519787729354928No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1190.1489398983704223No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1150.14393351523192072No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1130.14143032366266992No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1090.1364239405241683No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1060.13266915317029213No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1040.13016596160104132No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1030.1289143658164159No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1030.1289143658164159No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1020.12766277003179052No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1000.12515957846253972No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG950.11890159953941275No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG940.11765000375478735No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG870.10888883326240957No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG830.10388245012390798No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)