FastQCFastQC Report
Wed 8 May 2024
EGAF00008119125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44165
Total Bases13.2 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT19664.451488735423978No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13583.0748330125665118No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11912.696705536057965No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11292.5563228801086835No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11222.5404732254047326No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10612.4023548058417297No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8341.8883731461564586No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6871.5555303973734858No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6591.4921317785576813No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC5161.1683459753198233No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4350.9849428280312464No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4090.9260726819879996No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC3960.8966376089663761No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3750.8490886448545228No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3680.8332389901505717No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3530.7992754443563908No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3390.7675761349484886No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3340.756254953017095No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3200.7245556436091929No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3190.7222914072229141No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2960.6702139703385034No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2870.6498358428619948No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2850.6453073700894373No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2840.6430431337031586No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2660.6022868787501415No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2310.523038605230386No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2010.4551115136420242No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1980.44831880448318806No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1930.4369976225517944No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1870.42341220423412207No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1830.41435525868900713No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1810.40982678591644967No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1720.38944865843994114No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1640.3713347673497113No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1610.3645420581908751No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1470.33284274878297293No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1360.30793614853390694No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1320.29887920298879206No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1290.29208649382995583No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1270.2875580210573984No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1170.26491565719461113No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1120.2535944752632175No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1020.23095211140043023No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT960.21736669308275786No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT940.2128382203102004No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT940.2128382203102004No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC940.2128382203102004No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT930.21057398392392165No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC900.20378127476508548No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT870.19698856560624928No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC860.19472432921997057No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT850.19246009283369184No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC840.1901958564474131No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCTTGTAGTCCTGTCTCT820.18566738367485566No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT790.17887467451601946No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT760.1720819653571833No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCCAGTAGTCCTGTCTCT750.16981772897090455No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT750.16981772897090455No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT750.16981772897090455No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCGGGTAGTCCTGTCTCT750.16981772897090455No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT720.16302501981206838No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCGAGTAGTCCTGTCTCT710.16076078342578964No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT690.1562323106532322No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCCGGTAGTCCTGTCTCT680.15396807426695347No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC650.1471753651081173No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT650.1471753651081173No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT650.1471753651081173No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCTAGTAGTCCTGTCTCT630.14264689233555983No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT630.14264689233555983No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT630.14264689233555983No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCTGGTAGTCCTGTCTCT610.1381184195630024No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT600.13585418317672365No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT600.13585418317672365No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT600.13585418317672365No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT590.13358994679044495No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCGTGTAGTCCTGTCTCT590.13358994679044495No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT590.13358994679044495No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT570.12906147401788745No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT560.12679723763160874No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC560.12679723763160874No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT540.12226876485905128No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT530.12000452847277256No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT530.12000452847277256No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCCTGTAGTCCTGTCTCT520.11774029208649382No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT520.11774029208649382No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT520.11774029208649382No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT510.11547605570021512No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT510.11547605570021512No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT510.11547605570021512No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT500.11321181931393638No Hit
CCTACGGGTGGCTGCAGATGTGGATTAGATACCCGAGTAGTCCTGTCTCT500.11321181931393638No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT500.11321181931393638No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT500.11321181931393638No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT490.11094758292765763No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT490.11094758292765763No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT490.11094758292765763No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT490.11094758292765763No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT480.10868334654137893No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT480.10868334654137893No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT480.10868334654137893No Hit
CCTACGGGGGGCTGCAGATGTGGATTAGATACCCTGGTAGTCCTGTCTCT480.10868334654137893No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT480.10868334654137893No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT480.10868334654137893No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT470.1064191101551002No Hit
CCTACGGGTGGCAGCAGATGTGGATTAGATACCCTTGTAGTCCTGTCTCT470.1064191101551002No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT460.10415487376882146No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT460.10415487376882146No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT460.10415487376882146No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC460.10415487376882146No Hit
CCTACGGGCGGCAGCAGATGTGGATTAGATACCCCAGTAGTCCTGTCTCT460.10415487376882146No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC460.10415487376882146No Hit
CCTACGGGGGGCTGCAGATGTGGATTAGATACCCGGGTAGTCCTGTCTCT450.10189063738254274No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT450.10189063738254274No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)