FastQCFastQC Report
Wed 8 May 2024
EGAF00008119126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44165
Total Bases13.2 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG13723.1065323219744143No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG13623.083889958111627No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11162.5268878070870597No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10742.4317898788633534No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10402.3548058417298767No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10192.3072568776180233No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9542.160081512509906No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9452.1397033850333975No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8701.9698856560624929No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5201.1774029208649384No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4921.114004302049134No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4581.0370202649156572No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4511.021170610211706No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4501.0189063738254274No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4180.9464508094645082No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4100.9283369183742782No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4100.9283369183742782No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4050.9170157364428846No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3940.8921091361938187No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3650.8264462809917356No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3520.797011207970112No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3510.7947469715838333No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3380.7653118985622099No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3250.7358768255405865No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3200.7245556436091929No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3100.7019132797464055No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3060.6928563342012907No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1110.2513302388769388No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1060.2400090569455451No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT1020.23095211140043023No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1000.22642363862787276No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT920.20830974753764292No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT910.2060455111513642No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT900.20378127476508548No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG810.18340314728857693No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT790.17887467451601946No Hit
GACTACCCGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT770.17434620174346202No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG770.17434620174346202No Hit
GACTACAAGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT760.1720819653571833No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT760.1720819653571833No Hit
GACTACAGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT760.1720819653571833No Hit
GACTACTGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT740.16755349258462585No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG740.16755349258462585No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG730.1652892561983471No Hit
GACTACTCGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT700.1584965470395109No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG660.14943960149439603No Hit
GACTACACGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT660.14943960149439603No Hit
GACTACCAGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT650.1471753651081173No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG650.1471753651081173No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG650.1471753651081173No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT650.1471753651081173No Hit
GACTACTAGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT640.14491112872183856No Hit
GACTACCGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT630.14264689233555983No Hit
GACTACACGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT580.13132571040416618No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG570.12906147401788745No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT560.12679723763160874No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT560.12679723763160874No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT560.12679723763160874No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT540.12226876485905128No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT540.12226876485905128No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT520.11774029208649382No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT510.11547605570021512No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCT510.11547605570021512No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT500.11321181931393638No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT500.11321181931393638No Hit
GACTACAGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT500.11321181931393638No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT490.11094758292765763No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT490.11094758292765763No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT490.11094758292765763No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT480.10868334654137893No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA480.10868334654137893No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA470.1064191101551002No Hit
GACTACCAGGGTATCTAATCCACATCTGCAGCCCCCCGTAGGCTGTCTCT470.1064191101551002No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT460.10415487376882146No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG460.10415487376882146No Hit
GACTACAAGGGTATCTAATCCACATCTGCTGCCGCCCGTAGGCTGTCTCT460.10415487376882146No Hit
GACTACTAGGGTATCTAATCCACATCTGCTGCCACCCGTAGGCTGTCTCT460.10415487376882146No Hit
GACTACTCGGGTATCTAATCCACATCTGCAGCCACCCGTAGGCTGTCTCT460.10415487376882146No Hit
GACTACAAGGGTATCTAATCCACATCTGCTGCCACCCGTAGGCTGTCTCT460.10415487376882146No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT460.10415487376882146No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT450.10189063738254274No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG450.10189063738254274No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)