FastQCFastQC Report
Wed 8 May 2024
EGAF00008119129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60388
Total Bases18.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC12122.0070212625024837No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT9701.6062793932569386No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC8131.3462939656885473No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC7091.1740743194012055No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC7081.1724183612638273No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6991.1575147380274227No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6561.0863085381201563No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG6291.0415976684109425No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6231.0316619195866727No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6221.0300059614492947No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6041.0001987149764853No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5600.9273365569318407No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5540.9174008081075711No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5440.9008412267337882No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCG5390.8925614360468968No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5370.8892495197721402No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5070.8395707756507916No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4970.8230111942770086No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4720.7816122408425515No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4680.7749884082930383No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4140.6855666688746108No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4020.6656951712260715No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3980.6590713386765583No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3830.634231966615884No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3700.6127045108299662No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCG3680.6093925945552097No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3630.6011128038683182No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCG3600.5961449294561834No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG3600.5961449294561834No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG3540.5862091806319136No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3520.582897264357157No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3480.5762734318076439No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG3480.5762734318076439No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG3460.5729615155328873No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3380.559713850433861No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3370.5580578922964827No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3350.5547459760217262No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCG3320.5497781016095913No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3300.5464661853348348No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3290.5448102271974564No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG3210.5315625620984301No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCG3200.5299066039610518No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG3160.5232827714115387No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3140.5199708551367821No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3110.5150029807246473No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCG3020.5000993574882426No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2980.49347552493872954No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2940.4868516923892164No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2910.4818838179770815No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2900.48022785983970323No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2800.46366827846592035No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2750.45538848777902896No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2740.4537325296416507No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG2680.44379678081738094No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG2660.4404848645426244No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2650.438828906405246No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2630.4355169901304895No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCG2590.42889315758097635No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2530.4189574087567066No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2400.3974299529707889No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2140.3543750413989534No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2120.3510631251241969No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2110.34940716698681856No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2070.3427833344373054No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2050.33947141816254883No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG2030.3361595018877923No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2010.33284758561303573No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCG1940.3212558786513877No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1930.3195999205140094No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1910.3162880042392528No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG1830.30304033914022654No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1810.29972842286546997No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1800.2980724647280917No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG1560.2583294694310128No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1550.25667351129363447No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1550.25667351129363447No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG1540.25501755315625624No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1530.2533615950188779No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG1530.2533615950188779No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCG1520.25170563688149966No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1520.25170563688149966No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT1480.2450818043319865No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1450.24011392991985162No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG1410.2334900973703385No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1400.23183413923296017No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG1380.2285222229582036No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1380.2285222229582036No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1280.21196264158442074No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG1250.20699476717228588No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG1240.20533880903490762No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG1210.20037093462277272No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1190.19705901834801615No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG1160.1920911439358813No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG1140.18877922766112473No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1110.18381135324898987No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG1060.17553156256209845No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG1040.17221964628734185No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCG970.16062793932569386No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCG940.155660064913559No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCG900.14903623236404584No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT900.14903623236404584No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT890.14738027422666755No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC880.14572431608928926No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC860.1424123998145327No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCG850.1407564416771544No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT810.13413260912764124No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT810.13413260912764124No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT810.13413260912764124No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT790.13082069285288467No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT780.1291647347155064No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCG740.12254090216599325No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT740.12254090216599325No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG730.12088494402861497No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCG720.11922898589123666No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCG710.11757302775385838No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCG700.11591706961648009No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG690.1142611114791018No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT690.1142611114791018No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT690.1142611114791018No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC650.10763727892958866No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC650.10763727892958866No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT630.10432536265483208No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)