FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008119131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73993
Sequences flagged as poor quality0
Sequence length301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCG13141.7758436608868406No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG11351.5339288851648127No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC11171.5096022596732124No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10571.4285135080345437No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG8261.1163218142256701No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCG8081.0919951887340695No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCG7561.0217182706472232No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC7481.010906437095401No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCG7461.0082034787074452No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCG7401.0000946035435785No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCG7080.9568472693362886No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6970.9419809982025327No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG6900.932520643844688No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6810.9203573310988876No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6790.9176543727109321No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG6470.8744070385036422No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6370.8608922465638642No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6340.8568378089819308No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6290.8500804130120417No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG6210.8392685794602192No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6160.8325111834903303No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG6120.827105266714419No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG6110.8257537875204412No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5920.8000756828348627No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5870.7933182868649737No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5610.7581798278215507No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCG5610.7581798278215507No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5490.741962077493817No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG4780.6460070547213925No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG4750.6419526171394592No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCG4740.6406011379454813No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4580.6189774708418364No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG4570.6176259916478586No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4400.5946508453502358No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGACGGTAACTGACGGTGAGGC4270.5770816158285244No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4140.5595123863068128No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4110.5554579487248794No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4020.5432946359790791No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG3970.5365372400091901No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3920.529779844039301No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG3710.501398780965767No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG3540.4784236346681443No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3470.46896328031029966No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3430.46355736353438837No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG3430.46355736353438837No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG3420.4622058843404106No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG3340.45139405078858813No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3320.4486910924006325No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG3280.44328517562472125No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3270.44193369643074343No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCG3250.43923073804278784No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3200.4324733420728988No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3190.43112186287892096No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT3170.4284189044909654No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG2890.3905774870595867No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG2850.3851715702836755No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG2840.38382009108969767No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2790.37706269511980867No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2770.374359736731853No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2720.36760234076196396No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG2670.3608449447920749No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG2650.3581419864041193No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACGGTAACTGACGGTGAGGC2650.3581419864041193No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2620.3540875488221859No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGACGGTAACTGACGGTGAGGC2570.34733015285229685No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACGGTAACTGACGGTGAGGC2510.33922127768842997No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2460.33246388171854097No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCG2350.31759761058478503No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGACGGTAACTGACGGTGAGGC2330.31489465219682944No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGACGGTAACTGACGGTGAGGC2300.310840214614896No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2300.310840214614896No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG2300.310840214614896No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2290.3094887354209182No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2290.3094887354209182No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGACGGTAACTGACGGTGAGGC2280.3081372562269404No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG2240.30273133945102915No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT2190.2959739434811401No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2180.2946224642871623No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2150.29056802670522885No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACGGTAACTGACGGTGAGGC2130.28786506831727326No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCG2060.27840471395942856No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT1990.2689443596015839No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCG1980.2675928804076061No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1970.26624140121362827No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG1940.2621869636316949No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCG1920.25948400524373927No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG1920.25948400524373927No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1910.25813252604976145No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG1900.2567810468557836No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1890.2554295676618058No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG1860.25137513007987244No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCG1820.24596921330396118No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT1800.24326625491600554No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT1790.24191477572202777No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1770.23921181733407215No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG1690.22839998378224968No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1630.22029110861838283No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1620.21893962942440504No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1570.21218223345451595No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCG1540.20812779587258257No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1510.20407335829064913No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT1500.2027218790966713No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG1460.1973159623207601No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT1440.19461300393280445No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCG1350.1824496911870042No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1270.17163785763518172No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT1260.1702863784412039No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCG1220.16488046166529266No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1180.15947454488938143No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1180.15947454488938143No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1100.14866271133755896No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1100.14866271133755896No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1060.14325679456164772No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1040.1405538361736921No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1030.13920235697971428No Hit
CCTACGGGAGGCAGCAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAAT990.13379644020380305No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC980.13244496100982525No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC970.13109348181584746No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC940.12703904423391402No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC910.12298460665198059No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT900.12163312745800277No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT890.12028164826402499No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT870.11757868987606936No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT860.11622721068209153No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC820.11082129390618031No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT810.10946981471220252No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCG780.10541537713026908No Hit
CCTACGGGGGGCTGCAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAAT760.10271241874231346No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCG750.10136093954833565No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCG750.10136093954833565No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)