FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008119132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73993
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11221.5163596556431014No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10871.4690578838538781No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9501.2839052342789183No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9491.2825537550849404No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8951.2095738786101389No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8061.0892922303461137No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7781.051450812914735No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7641.0325301041990458No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7591.0257727082291568No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7421.002797561931534No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6820.9217088102928656No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6640.897382184801265No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6530.882515913667509No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6410.8662981633397755No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6050.8176449123565743No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5820.7865608908950846No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5800.7838579325071291No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5750.77710053653724No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5640.762234265403484No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5630.7608827862095063No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG5630.7608827862095063No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5260.7108780560323275No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5260.7108780560323275No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG5060.6838484721527712No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG5030.6797940345708378No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4920.6649277634370819No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4920.6649277634370819No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4880.6595218466611706No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG4870.6581703674671928No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4840.6541159298852595No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4770.6446555755274147No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG4520.6108685956779696No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG4170.5635668238887462No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG4130.5581609071128351No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3970.5365372400091901No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG3880.5243739272633898No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG3810.5149135729055452No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG3620.4892354682199667No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG3370.4554484883705215No Hit
GACTACTGGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG3360.4540970091765437No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3220.4351763004608544No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG3200.4324733420728988No Hit
GACTACCGGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG3180.4297703836849432No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG3150.4257159461030098No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG3120.4216615085210763No Hit
GACTACTCGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG3100.41895855013312067No Hit
GACTACAAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG3020.40814671658129825No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2910.3932804454475423No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG2900.39192896625356455No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAC2890.3905774870595867No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAC2860.3865230494776533No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2860.3865230494776533No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2810.37976565350776426No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG2790.37706269511980867No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG2780.37571121592583084No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2780.37571121592583084No Hit
GACTACCCGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG2740.37030529914991955No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAC2690.36354790318003055No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG2690.36354790318003055No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2670.3608449447920749No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAC2650.3581419864041193No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAC2640.3567905072101415No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2630.3554390280161637No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAC2540.34327571527036344No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2520.3405727568824078No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2500.3378697984944522No Hit
GACTACCAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG2490.3365183193004744No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2460.33246388171854097No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2370.3203005689727407No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2370.3203005689727407No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2350.31759761058478503No Hit
GACTACAGGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG2350.31759761058478503No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAC2290.3094887354209182No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2210.29867690186909573No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2200.2973254226751179No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2190.2959739434811401No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2180.2946224642871623No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2140.28921654751125103No Hit
GACTACACGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG2130.28786506831727326No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2110.2851621099293177No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAC2060.27840471395942856No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2060.27840471395942856No Hit
GACTACTAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG2060.27840471395942856No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1990.2689443596015839No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAC1990.2689443596015839No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1990.2689443596015839No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1970.26624140121362827No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1930.2608354844377171No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1790.24191477572202777No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1790.24191477572202777No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1780.24056329652804997No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1730.2338059005581609No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1630.22029110861838283No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1620.21893962942440504No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1580.21353371264849377No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1540.20812779587258257No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1490.20137039990269348No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG1480.2000189207087157No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1440.19461300393280445No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1400.18920708715689322No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1390.18785560796291542No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCCTTCGTGCTTCAG1340.18109821199302636No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1330.17974673279904854No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1320.17839525360507075No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1310.17704377441109295No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1300.17569229521711513No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCACGCCTTCGTGCTTCAG1290.1743408160231373No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG1280.1729893368291595No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1280.1729893368291595No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG1270.17163785763518172No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1260.1702863784412039No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1240.16758342005324828No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1240.16758342005324828No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCATGAG1230.16623194085927048No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1230.16623194085927048No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1220.16488046166529266No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1220.16488046166529266No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1200.16217750327733704No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG1200.16217750327733704No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1190.16082602408335925No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1160.1567715865014258No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCATGAG1120.15136566972551457No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCCTTCGTGCTTCAG1090.14731123214358116No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCACGCCTTCGTGCTTCAG1090.14731123214358116No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG1050.14190531536766993No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCACGCCTTCGTGCTTCAG1030.13920235697971428No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1020.1378508777857365No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1020.1378508777857365No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG1010.1364993985917587No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG990.13379644020380305No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCACGCCTTCGTGCTTCAG980.13244496100982525No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG970.13109348181584746No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCACGCCTTCGTGCTTCAG970.13109348181584746No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA930.12568756503993622No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCATGAG910.12298460665198059No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG870.11757868987606936No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG860.11622721068209153No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCATGAG850.11487573148811375No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCACGCCTTCGTGCTTCAG840.11352425229413593No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG840.11352425229413593No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG840.11352425229413593No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA840.11352425229413593No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCATGAG830.11217277310015812No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG810.10946981471220252No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG810.10946981471220252No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCATGAG800.1081183355182247No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG790.10676685632424689No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG780.10541537713026908No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCATGAG780.10541537713026908No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG770.10406389793629128No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCATGAG750.10136093954833565No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCCTTCGTGCTTCAG750.10136093954833565No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG750.10136093954833565No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG740.10000946035435784No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG740.10000946035435784No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)