FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008119136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147711
Sequences flagged as poor quality0
Sequence length301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG59934.057246921353183No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG58563.964498243191096No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG45153.0566443934439547No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG42572.8819789995328717No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG39422.6687247395251537No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG34772.353920831894713No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG32872.2252912782392644No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG30402.058072858487181No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG26041.762901882730467No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13150.8902519108258695No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12410.8401540846653263No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10220.691891599136151No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9190.6221608411018814No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8250.5585230619249751No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7290.49353128744643254No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7140.4833763226841603No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7140.4833763226841603No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7120.482022327382524No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7080.4793143367792514No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6720.45494242134979795No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6400.43327849652361705No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCNCCACGCTTTCGCTCCTCAG6130.414999559951527No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCNCCACGCTTTCGCTCCTCAG6090.41229156934825434No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5910.40010561163352765No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5430.3676097243942563No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5130.3472997948697118No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5110.3459457995680755No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5080.343914806615621No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4790.3242818747418946No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4690.3175118982337131No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4520.30600293816980456No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4490.3039719452173501No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4260.2884009992485326No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4230.2863700062960781No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCNCCACGCTTTCGCTCCTCAG4100.2775690368354422No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCNCCACGCTTTCGCTCCTCAG4070.27553804388298775No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3980.2694450650256244No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3830.2592901002633521No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG3790.2565821096600795No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3690.24981213315189796No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG3600.24371915429453458No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3470.23491818483389865No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCNCCACGCTTTCGCTCCTCAG3460.23424118718308046No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3410.23085619892898973No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3340.22611721537326265No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCNCCACGCTTTCGCTCCTCAG3340.22611721537326265No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3180.2152852529601722No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3140.2125772623568996No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCNCCACGCTTTCGCTCCTCAG2990.20242229759462735No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2980.20174529994380921No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCNCCACGCTTTCGCTCCTCAG2930.19836031168971843No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2930.19836031168971843No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG2890.1956523210864458No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2850.19294433048317322No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2660.18008137511762834No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG2520.1706034080061742No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2470.16721841975208346No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2450.16586442445044716No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG2380.16112544089472008No Hit
GACTACTCGGGTATCTAATCCTGTTCGNTCCCCACGCTTTCGCTCCTCAG2370.16044844324390195No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCNCCACGCTTTCGCTCCTCAG2370.16044844324390195No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2340.1584174502914475No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG2330.15774045264062936No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2290.1550324620373567No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2290.1550324620373567No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG2280.15435546438653858No Hit
GACTACTCGGGTATCTAATCCTGTTCGNTCNCCACGCTTTCGCTCCTCAG2210.14961648083081153No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG2140.14487749727508445No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2140.14487749727508445No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG2120.14352350197344815No Hit
GACTACTGGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG2070.1401385137193574No Hit
GACTACTGGGGTATCTAATCCTGTTCGNTCNCCACGCTTTCGCTCCTCAG2050.1387845184177211No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2040.13810752076690294No Hit
GACTACTGGGGTATCTAATCCTGTTCGNTCCCCACGCTTTCGCTCCTCAG2020.13675352546526665No Hit
GACTACTCGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG1990.1347225325128122No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG1980.13404553486199403No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1910.12930655130626698No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1860.12592156305217622No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1860.12592156305217622No Hit
GACTACTGGGGTATCTAATNCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1810.12253657479808545No Hit
GACTACTCGGGTATCTAATNNTGTTCGCTCCCCACGCTTTCGCTCCTCAG1790.12118257949644914No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1790.12118257949644914No Hit
GACTACTAGGGTATCTAATCCTGTTCGNTCNCCACGCTTTCGCTCCTCAG1770.11982858419481285No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1770.11982858419481285No Hit
GACTACCGGGGTATCTAATCCTGTTCGNTCNCCACGCTTTCGCTCCTCAG1770.11982858419481285No Hit
GACTACTGGGGTATCTAATNNTGTTCGCTCCCCACGCTTTCGCTCCTCAG1760.11915158654399469No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG1750.11847458889317655No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1730.11712059359154023No Hit
GACTACTCGGGTATCTAATNCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1710.11576659828990393No Hit
GACTACTAGGGTATCTAATCCTGTTCGNTCCCCACGCTTTCGCTCCTCAG1690.11441260298826762No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1660.11238161003581316No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG1640.11102761473417687No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG1580.10696562882926797No Hit
GACTACCCGGGTATCTAATCCTGTTCGNTCCCCACGCTTTCGCTCCTCAG1540.10425763822599536No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1530.1035806405751772No Hit
GACTACCGGGGTATCTAATNNTGTTCGCTCCCCACGCTTTCGCTCCTCAG1530.1035806405751772No Hit
GACTACCGGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG1510.1022266452735409No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1500.10154964762272274No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG1500.10154964762272274No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)