FastQCFastQC Report
Wed 9 Aug 2023
EGAF00008119165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63388
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT21493.390231589575314No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT14312.25752508361204No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT14272.2512147409604344No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT13862.186533728781473No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11231.7716286994383794No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9541.5050167224080269No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA9101.435602953240361No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7391.1658358048842052No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7251.1437496056035843No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA6941.094844450053638No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA6501.0254306808859721No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT5960.9402410550892912No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5660.8929134852022464No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5070.7998359310910582No Hit
CCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT4380.6909825203508551No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT4300.678361835047643No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4170.6578532214299236No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3970.6263015081718938No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3960.6247239225089922No Hit
CCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT3180.5016722408026756No Hit
CCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT3100.4890515554994636No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3080.48589638417366066No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2980.4701205275446456No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2930.46223259923013815No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA2900.45749984224143375No Hit
CCTACGGGCGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT2680.42279295765760083No Hit
CCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT2490.39281883006247237No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2450.3865084874108664No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2350.3707326307818515No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2140.33760333186092No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA2050.3234050608948066No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA1990.3139395469173976No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1770.2792326623335647No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1640.25872404871584526No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA1630.25714646305294375No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1630.25714646305294375No Hit
CCTACGGGCGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT1580.2492585347384363No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA1440.22717233545781534No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1360.2145516501546034No Hit
CCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT1350.2129740644917019No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1310.20666372184009593No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1310.20666372184009593No Hit
CCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT1280.20193096485139145No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1280.20193096485139145No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA1210.19088786521108098No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1200.18931027954817947No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1060.16722408026755853No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1060.16722408026755853No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT1050.16564649460465702No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1050.16564649460465702No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA1020.16091373761595254No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1010.15933615195305106No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT990.15618098062724806No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT980.15460339496434658No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT980.15460339496434658No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT880.13882753833533162No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT850.1340947813466271No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC840.13251719568372564No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT840.13251719568372564No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT820.12936202435792263No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT810.12778443869502115No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT810.12778443869502115No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC800.12620685303211962No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT770.12147409604341516No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA760.11989651038051366No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT750.11831892471761217No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT730.11516375339180916No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT720.11358616772890767No Hit
CCTACGGGTGGCTGCAGTGGCGAAGGCGGATTGCTGGACAGTAACTGACG710.11200858206600618No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT700.11043099640310469No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT680.1072758250773017No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT680.1072758250773017No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC650.10254306808859721No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG650.10254306808859721No Hit
CCTACGGGTGGCTGCAGTAGCGAAGGCGGCTTTCTGGACTGTAACTGACG650.10254306808859721No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)