FastQCFastQC Report
Wed 8 May 2024
EGAF00008119173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68393
Total Bases20.5 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT15712.2970187007442284No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10641.5557147661310369No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10201.4913806968549412No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9651.410963110259822No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7881.1521646952173468No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7231.0571257292412966No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5440.7954030383226354No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4930.7208340034798884No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4170.6097115201848142No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3960.5790066234848595No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3860.5643852441039288No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3530.5161346921468571No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3470.5073618645182987No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3020.4415656573041101No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2810.4108607606041554No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2760.40355007091369005No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2720.3977015191613177No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2620.3830801397803869No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2530.36992089833754915No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2460.3596859327708976No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2330.3406781395756876No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2260.330443174009036No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2240.32751889813284984No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2210.3231324843185706No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2140.31289751875191907No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2140.31289751875191907No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2120.3099732428757329No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2070.3026625531852675No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2060.3012004152471744No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1910.2792683461757782No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1880.274881932361499No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1830.26757124267103355No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1790.26172269091866124No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1740.2544120012281959No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1660.2427148977234512No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1570.22955565628061353No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1560.22809351834252042No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1550.22663138040442737No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1530.22370710452824122No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1460.21347213896158965No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1400.20469931133303118No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1390.20323717339493808No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1380.20177503545684503No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1370.20031289751875192No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1370.20031289751875192No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1360.19885075958065884No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1320.19300220782828653No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1270.18569151813782112No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1260.18422938019972804No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1260.18422938019972804No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1260.18422938019972804No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1240.18130510432354188No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1230.1798429663854488No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1210.17691869050926262No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1180.1725322766949834No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1150.16814586288070416No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1140.16668372494261108No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1100.16083517319023877No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1090.1593730352521457No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1080.1579108973140526No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT1050.15352448349977338No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1030.1506002076235872No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1030.1506002076235872No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1030.1506002076235872No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1020.14913806968549412No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1020.14913806968549412No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC950.13890310411884257No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT940.1374409661807495No Hit
CCTACGGGTGGCTGCAGTAGCGAAGGCGGCTTTCTGGACTGTAACTGACG940.1374409661807495No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT930.13597882824265642No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT910.13305455236647026No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT910.13305455236647026No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT900.13159241442837719No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT870.12720600061409795No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT850.12428172473791178No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT850.12428172473791178No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT840.12281958679981869No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT820.11989531092363252No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT810.11843317298553944No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC790.11550889710935329No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT770.11258462123316713No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT760.11112248329507406No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT760.11112248329507406No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC750.10966034535698098No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT750.10966034535698098No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT750.10966034535698098No Hit
CCTACGGGTGGCTGCAGTGGCGAAGGCGGATTGCTGGACGACAACTGACG720.10527393154270175No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT720.10527393154270175No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT710.10381179360460867No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC700.10234965566651559No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG690.10088751772842251No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)