FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008119206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119958
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG29142.4291835475749846No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG26162.1807632671435No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG22581.8823254805848715No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG21301.7756214675136297No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG19731.644742326480935No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG19711.6430750762766968No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG18241.5205321862651928No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG17301.4421714266659997No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG15601.300455159305757No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG15291.2746127811400656No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG14661.222094399706564No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13831.1529035162306807No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG13251.1045532603077743No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12431.0361960019340102No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11890.9911802464195802No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11820.9853448707047466No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10350.8628019806932427No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG10120.843628603344504No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9650.8044482235449074No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8760.7302555894563096No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7740.6452258290401641No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6980.5818703212791144No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6960.5802030710748762No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6580.5485253171943514No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6240.5201820637223028No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6210.5176811884159456No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6110.5093449373947548No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6000.5001750612714451No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5740.4785008086163491No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5530.46099468147184847No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5460.45515930575701496No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5350.44598942963370514No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5100.42514880208072825No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5030.41931342636589475No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5020.41847980126377565No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4990.41597892595741837No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4840.40347454942563227No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4540.37846579636206007No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4490.3742976708514647No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4460.3717967955451075No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4340.36179329431967855No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG4180.34845529268577335No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4120.3434535420730589No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4090.34095266676670166No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3970.3309491655412728No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3900.32511378982643924No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3850.32094566431584387No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3810.31761116390736754No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3810.31761116390736754No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3720.3101085379882959No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3660.30510678737558145No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3660.30510678737558145No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3620.3017722869671051No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG3610.3009386618649861No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3520.2934360359459144No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG3410.28426615982260456No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3390.2825989096183664No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3260.27176178329081846No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3160.2634255322696277No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3130.2609246569632705No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3090.25759015655479417No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3070.25592290635055603No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3040.2534220310441988No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG2950.2459194051251271No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG2950.2459194051251271No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2910.24258490471665084No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2850.23758315410393635No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2790.23258140349122192No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2700.22507877757215025No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2400.200070024508578No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2390.19923639940645893No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2350.19590189899798263No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2280.1900665232831491No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG2270.18923289818103003No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2240.1867320228746728No Hit
GACTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2220.18506477267043467No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2200.1833975224661965No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG2170.18089664715983927No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG2160.1800630220577202No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2130.17756214675136298No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2110.17589489654712484No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2050.17089314593441038No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2000.16672502042381498No Hit
GACTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1990.16589139532169592No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1940.16172326981110055No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG1940.16172326981110055No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1850.15422064389202889No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1840.15338701878990982No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1820.15171976858567165No Hit
GACTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1780.14838526817719536No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1730.14421714266659996No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1600.133380016339052No Hit
GACTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1490.12421014021574217No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1450.12087563980726587No Hit
GACTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1450.12087563980726587No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1420.11837476450090864No Hit
GACTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1400.11670751429667049No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1380.11504026409243236No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1350.11253938878607513No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1300.10837126327547975No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1300.10837126327547975No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1220.10170226245852715No Hit
GACTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1210.10086863735640807No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1210.10086863735640807No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1200.100035012254289No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)