FastQCFastQC Report
Wed 8 May 2024
EGAF00008119213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122156
Total Bases36.7 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT29922.4493270899505553No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT24331.9917155113134026No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT22711.8590982022987No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT21181.733848521562592No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT20081.6437997314908808No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT17371.4219522577687547No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT15641.2803300697468811No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT15551.2729624414682865No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT14931.2222076688824126No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12020.9839876878745211No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11230.9193162840957465No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11160.9135859065457284No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10190.8341792462097646No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9950.8145322374668456No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8750.7162971937522512No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7890.6458954124234586No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5530.4526998264514227No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5440.44533219817282815No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT5170.4232293133370445No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT4910.401945053865549No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4460.36510691247257604No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC4440.3634696617439995No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC4330.3544647827368283No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3920.32090114280100857No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3820.3127148891581257No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3640.2979796326009365No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3410.2791512492223059No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3310.27096499557942305No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3270.26769049412226986No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3170.25950424047938697No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3160.25868561511509874No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3080.2521366122007924No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3060.25049936147221585No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2910.23821998100789157No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2890.23658273027931495No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2770.22675922590785552No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2620.21447984544353121No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2580.21120534398637808No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2510.2054749664363601No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2450.20056321425063034No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2420.1981073381577655No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT2370.19401421133632404No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT2250.18419070696486461No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT2220.18173483087199974No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2220.18173483087199974No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT2200.18009758014342317No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2140.17518582795769344No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2100.1719113265005403No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2060.16863682504338715No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2060.16863682504338715No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT2030.16618094895052227No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT2030.16618094895052227No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2000.16372507285765744No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1990.16290644749336913No Hit
CCTACGGGTGGCTGCAGTAGCGAAGGCGGCTTTCTGGACTGTAACTGACG1990.16290644749336913No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1970.16126919676479257No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1780.1457153148433151No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1780.1457153148433151No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1770.1448966894790268No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1760.14407806411473853No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1750.14325943875045025No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1730.14162218802187368No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1710.13998493729329708No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT1710.13998493729329708No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1650.13507318510756736No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1650.13507318510756736No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGGT1610.13179868365041422No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1580.12934280755754937No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1550.1268869314646845No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1530.12524968073610793No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1500.12279380464324306No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT1490.12197517927895478No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1480.1211565539146665No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1470.12033792855037821No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT1460.11951930318608991No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1460.11951930318608991No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1450.11870067782180163No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT1430.11706342709322506No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG1430.11706342709322506No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1410.11542617636464847No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1400.1146075510003602No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1380.11297030027178363No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1330.10887717345034219No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1330.10887717345034219No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1320.10805854808605389No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1320.10805854808605389No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1320.10805854808605389No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1310.10723992272176561No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1310.10723992272176561No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1310.10723992272176561No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1310.10723992272176561No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1310.10723992272176561No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1310.10723992272176561No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1300.10642129735747732No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1260.10314679590032418No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1260.10314679590032418No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT1240.10150954517174761No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1230.10069091980745933No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)