FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008119214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122156
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC22421.8353580667343397No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC19971.6347948524837095No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG18031.4759815318117815No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG17051.3957562461115296No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC16251.3302662169684665No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC15041.231212547889584No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC14471.1845509021251515No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG14091.1534431382821964No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG13871.1354333802678542No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC13171.0781296047676743No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG12231.001178820524575No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC11090.9078555289957104No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG10510.8603752578669898No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10390.8505517534955302No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9680.792429352631062No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9060.7416745800451882No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8770.7179344444808278No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7440.6090572710304857No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4940.4044009299584138No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4580.3749304168440355No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4540.37165591538688236No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4410.3610137856511346No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4290.35119028127967516No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4250.34791577982252203No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4150.33972952617963914No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4140.33891090081535086No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4050.3315432725367563No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4020.32908739644389146No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3740.3061658862438194No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3420.27996987458659417No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3300.2701463702151347No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3180.2603228658436753No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3130.2562297390222339No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3100.253773862929369No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3080.2521366122007924No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3040.2488621107436393No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2960.242313107829333No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2830.23167097809358525No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2730.2234847244507024No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2680.21939159762926094No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2600.21284259471495465No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2590.21202396935066634No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2570.21038671862208977No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2570.21038671862208977No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2470.2022004649792069No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2430.19892596352205377No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2350.19237696060774748No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT2340.1915583352434592No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2330.19073970987917088No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2260.1850093323291529No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2170.1776417040505583No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT2130.17436720259340516No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT2090.171092701136252No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT2080.1702740757719637No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2030.16618094895052227No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2020.16536232358623398No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2020.16536232358623398No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT2000.16372507285765744No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1960.16045057140050428No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1950.15963194603621597No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1920.15717606994335112No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT1890.15472019385048627No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1880.153901568486198No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1880.153901568486198No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1870.1530829431219097No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1860.15226431775762142No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1860.15226431775762142No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1810.14817119093617998No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1710.13998493729329708No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT1710.13998493729329708No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1690.13834768656472052No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1660.13589181047185564No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1660.13589181047185564No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT1660.13589181047185564No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT1650.13507318510756736No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1600.13098005828612594No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT1600.13098005828612594No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1590.13016143292183766No Hit
GACTACTCGGGTATCTAATCCGAAGACCTCCTTCCTCCACGCGGCGTCGC1560.12770555682897278No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1530.12524968073610793No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1510.12361243000753135No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1500.12279380464324306No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT1500.12279380464324306No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1500.12279380464324306No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1500.12279380464324306No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1500.12279380464324306No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1480.1211565539146665No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT1440.11788205245751335No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCTGCGTCGC1430.11706342709322506No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1420.11624480172893675No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT1420.11624480172893675No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT1410.11542617636464847No Hit
GACTACTCGGGTATCTAATCCGAAGACCCTCTTCCTCCACGCGGCGTCGC1410.11542617636464847No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1410.11542617636464847No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1410.11542617636464847No Hit
GACTACTGGGGTATCTAATCCGAAGACCCTCTTCCTCCACGCGGCGTCGC1400.1146075510003602No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG1390.11378892563607192No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT1390.11378892563607192No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCCTCACGCGGCATTGC1380.11297030027178363No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG1370.11215167490749532No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCTCCCGTAGGCTGTCTCT1370.11215167490749532No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1350.11051442417891875No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT1340.10969579881463047No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1310.10723992272176561No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCAGCCTCCCGTAGGCTGTCTCT1300.10642129735747732No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT1300.10642129735747732No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1290.10560267199318904No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT1280.10478404662890076No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCTCCCGTAGGCTGTCTCT1270.10396542126461246No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1260.10314679590032418No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCCTCACGCGGCATTGC1250.10232817053603589No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCTCCCGTAGGCTGTCTCT1250.10232817053603589No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCTGCGTCGC1230.10069091980745933No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)