FastQCFastQC Report
Wed 8 May 2024
EGAF00008119226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104507
Total Bases31.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA63176.044571177050341No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA61575.891471384691934No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA50044.788196006009167No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA48394.63031184513956No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA40693.8935190944147284No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA38213.656214416259198No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA36223.46579654951343No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA31633.0265915201852507No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA28602.7366587884065185No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7000.6698115915680289No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6390.6114422957313864No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6370.6095285483269064No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6160.5894342005798654No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5620.5377630206589032No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5540.5301080310409828No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5470.5234099151253027No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5310.508099935889462No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5280.5052293147827418No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5000.4784368511200207No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4890.4679112403953802No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4740.45355813486177954No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4740.45355813486177954No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4680.44781689264833935No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4400.42102442898561815No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4080.39040447051393684No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4060.3884907231094568No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4060.3884907231094568No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3980.38083573349153643No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3830.36648262795793585No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3740.35787076463777545No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3660.3502157750198551No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3630.34734515391313503No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3600.3444745328064149No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3590.34351765910417487No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3510.3358626694862545No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3350.32055269025041383No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3270.3128977006324935No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3230.30907020582353334No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3100.29663084769441284No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3020.2889758580764925No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2870.27462275254289187No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2850.2727090051384118No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2840.2717521314361717No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2730.26122652071153124No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2630.2516577836891309No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2610.24974403628465078No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2600.24878716258241074No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2430.23252030964433004No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2330.2229515726219296No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2070.19807285636368857No Hit
GACTACTGGGGTATCTAATCNTGTTTGCTACCCACGCTTTCGAGCATGAA1950.18659037193680805No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1920.18371975083008793No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATCAA1910.1827628771278479No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGNTTTCGAGCATGAA1850.17702163491440764No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATCAA1720.1645822767852871No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATCAA1670.1597979082740869No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1660.15884103457184687No Hit
GACTACTCGGGTATCTAATCNTGTTTGCTACCCACGCTTTCGAGCATGAA1650.15788416086960683No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1580.1511860449539265No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGNTTTCGAGCATGAA1510.14448792903824625No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGNTTTCGAGCATGAA1440.13778981312256594No Hit
GACTACCCGGGTATCTAATCNTGTTTGCTACCCACGCTTTCGAGCATGAA1440.13778981312256594No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1420.13587606571808586No Hit
GACTACCGGGGTATCTAATCNTGTTTGCTACCCACGCTTTCGAGCATGAA1360.1301348235046456No Hit
GACTACCAGGGTATCTAATCNTGTTTGCTACCCACGCTTTCGAGCATGAA1290.12343670758896534No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGNTTTCGAGCATGAA1280.12247983388672529No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGNTTTCGAGCATGAA1240.11865233907776512No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATCAA1210.11578171797104501No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATCAA1150.11004047575760476No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGNTTTCGAGCATGAA1140.10908360205536471No Hit
GACTACTAGGGTATCTAATCNTGTTTGCTACCCACGCTTTCGAGCATGAA1130.10812672835312467No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGNTTTCGAGCATGAA1090.10429923354416451No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1070.10238548613968443No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)