FastQCFastQC Report
Wed 8 May 2024
EGAF00008119232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114370
Total Bases34.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG46144.034274722392236No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG44253.869021596572528No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG35023.0619917810614674No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG33492.92821544111218No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG29602.588091282679024No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG27492.403602343271837No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG26612.3266590889219203No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG22651.9804144443472937No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG19311.6883798198828366No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG9590.8385066013814813No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG9240.8079041706741279No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9200.8044067500218589No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8380.7327096266503453No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG7530.6583894377896302No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG7490.6548920171373612No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6450.5639590801783685No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6430.5622103698522339No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6380.5578385940368978No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5730.5010055084375273No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5670.4957593774591239No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5460.47739791903471196No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5450.47652356387164463No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5100.44592113316429133No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5020.43892629185975346No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4630.4048264405001311No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4530.3960828888694588No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4400.38471627174958467No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG4240.3707265891405089No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG4150.36285739267290373No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3970.34711899973769345No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3920.3427472239223573No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3900.3409985135962228No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3790.33138060680248316No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3470.30340124158433157No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG3310.2894115589752558No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG3090.2701757453877765No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3050.26667832473550757No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3000.26230654892017136No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2990.26143219375710414No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2910.25443735245256627No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2830.24744251114802834No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG2800.24481944565882663No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2730.23869895951735598No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG2720.23782460435428873No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG2620.22908105272361634No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2430.21246830462533883No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2280.19935297717933023No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2120.18536329457025444No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2090.18274022908105272No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG2090.18274022908105272No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG1920.16787619130890968No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1910.16700183614584246No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1880.16437877065664072No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1840.1608813500043718No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1810.15825828451517007No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1810.15825828451517007No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1780.15563521902596833No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG1740.1521377983736994No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1730.15126344321063215No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1680.14689166739529597No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1680.14689166739529597No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1610.14077118125382532No Hit
GACTACTGGGGTATCTAATNCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1530.1337763399492874No Hit
GACTACTGGGGTATCTAATCCTGTTTGNTCNCCACGCTTTCGAGCCTCAG1520.13290198478622015No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG1500.13115327446008568No Hit
GACTACTGGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGAGCCTCAG1500.13115327446008568No Hit
GACTACTCGGGTATCTAATNCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1460.12765585380781674No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1460.12765585380781674No Hit
GACTACTGGGGTATCTAATCCTGTTTGNTCCCCACGCTTTCGAGCCTCAG1440.12590714348168225No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1420.12415843315554777No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1410.12328407799248055No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1390.12153536766634607No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1390.12153536766634607No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1380.12066101250327882No Hit
GACTACTGGGGTATCTAATNNTGTTTGCTCCCCACGCTTTCGAGCCTCAG1370.11978665734021159No Hit
GACTACTCGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGAGCCTCAG1350.11803794701407712No Hit
GACTACTCGGGTATCTAATCCTGTTTGNTCCCCACGCTTTCGAGCCTCAG1340.11716359185100989No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1330.11628923668794264No Hit
GACTACTCGGGTATCTAATNNTGTTTGCTCCCCACGCTTTCGAGCCTCAG1310.11454052636180817No Hit
GACTACTCGGGTATCTAATCCTGTTTGNTCNCCACGCTTTCGAGCCTCAG1300.11366617119874094No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1280.11191746087260644No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1200.10492261956806856No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG1190.10404826440500131No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1190.10404826440500131No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1180.10317390924193406No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1180.10317390924193406No Hit
GACTACCGGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGAGCCTCAG1170.10229955407886683No Hit
GACTACCGGGGTATCTAATNNTGTTTGCTCCCCACGCTTTCGAGCCTCAG1160.10142519891579961No Hit
GACTACCGGGGTATCTAATNCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1160.10142519891579961No Hit
GACTACCGGGGTATCTAATCCTGTTTGNTCNCCACGCTTTCGAGCCTCAG1160.10142519891579961No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)