FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008119259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44137
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT27226.1671613385594855No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT24775.612071504633301No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT20424.626503840315381No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC20194.574393366110066No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT18284.141649862926796No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC15593.532183882003761No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT15543.5208555180460834No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT15193.441556970342343No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC14503.2852255477263976No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC11842.6825565851779687No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC10892.4673176699821013No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT10302.3336429752815095No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC8882.011917438883476No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC7571.7151143031923328No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7441.6856605569023722No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4791.085257267145479No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3900.8836123886988241No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3440.7793914402881936No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3400.7703287491220517No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3110.7046242381675238No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2480.5618868523007907No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2370.5369644515939008No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2300.5211047420531527No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2260.5120420508870109No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2250.5097763780954755No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1890.42821215760019943No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1800.40782110247638037No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1600.3625076466456714No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1570.3557106282710651No Hit
ACTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1200.2718807349842536No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1140.2582866982350409No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1100.24922400706889913No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1000.22656727915354463No Hit
ACTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT980.22203593357047374No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC950.2152389151958674No Hit
ACTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT900.20391055123819019No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC880.1993792056551193No Hit
ACTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT770.17445680494822938No Hit
ACTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC770.17445680494822938No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGCAAGCCTGAC750.1699254593651585No Hit
CATACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT710.16086276819901668No Hit
ACTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC700.15859709540748126No Hit
ACTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT680.15406574982441035No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC620.14047171307519768No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGCAAGCCTGAC610.13820604028366223No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC580.1314090219090559No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC570.12914334911752046No Hit
CATACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT550.12461200353444957No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC550.12461200353444957No Hit
CATACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC550.12461200353444957No Hit
ACTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC540.12234633074291411No Hit
ACTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC540.12234633074291411No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC530.12008065795137866No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC520.11781498515984323No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGCAAGCCTGAC500.11328363957677232No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC490.11101796678523687No Hit
ACTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT470.10648662120216598No Hit
CATACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT460.10422094841063052No Hit
CATACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC460.10422094841063052No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGCAAGCCTGAC450.10195527561909509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)