FastQCFastQC Report
Wed 8 May 2024
EGAF00008119271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95084
Total Bases28.6 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT37583.952294813007446No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT29593.1119851920407218No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT25512.682890917504522No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT23912.5146186529805226No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT21722.284295990913298No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT20052.1086618148163727No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT16281.7121702915316985No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT14331.5070884691430735No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT13621.4324176517605485No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12671.3325059946994238No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12621.3272474864330486No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT10661.1211139623911488No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10311.084304404526524No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8640.9086702284295991No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6650.6993815994278743No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6060.6373312018846494No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT5510.5794876109545244No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT4190.44066299272222453No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3790.39859492659122464No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3460.3638887720331496No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3440.36178536872659967No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2930.3081485844095747No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2920.30709688275629965No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2740.2881662529973497No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2700.28395944638424975No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2670.28080434142442473No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2490.26187371166547474No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2370.24925329182617476No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT2370.24925329182617476No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2150.22611585545412477No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2020.21244373396154978No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1980.20823692734844979No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1950.2050818223886248No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1940.20403012073534982No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGCTCTCTGGTCTGTAACTGACG1880.19771991081569978No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1870.1966682091624248No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG1800.18930629758949982No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1660.17458247444364983No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGCTCTCTGGTCTGTAACTGACG1640.17247907113709982No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1630.1714273694838248No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1600.16827226452399985No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1590.16722056287072484No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1560.16406545791089985No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1550.16301375625762485No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1530.16091035295107484No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1510.15880694964452483No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1480.15565184468469984No Hit
CCTACGGGCGGCTGCAGTGGGGAAGGCGGCTCTCTGGTCTGTAACTGACG1420.14934163476504986No Hit
CCTACGGGCGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG1410.14828993311177485No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT1410.14828993311177485No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1400.14723823145849987No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1370.14408312649867486No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1330.13987631988557486No Hit
CCTACGGGTGGCTGCAGTGGTGAAGGCGGATTGCTGGACAGTAACTGACG1330.13987631988557486No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1310.13777291657902485No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1270.13356610996592486No Hit
CCTACGGGTAGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1270.13356610996592486No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1260.13251440831264985No Hit
CCTACGGGTGGCAGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG1260.13251440831264985No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG1230.12935930335282486No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1230.12935930335282486No Hit
CCTACGGGCGGCTGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1220.12830760169954988No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1210.12725590004627488No Hit
CCTACGGGTGGCTGCAGTCGTCGGCGGCGTCAGATGTGTTATAACCGGAT1180.12410079508644989No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1150.12094569012662487No Hit
CCTACGGGTGGCAGCAGTGGGGAAGGCGGCTCTCTGGTCTGTAACTGACG1140.11989398847334987No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1120.11779058516679988No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1110.11673888351352488No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1100.11568718186024989No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1090.11463548020697488No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1080.11358377855369989No Hit
CCTACGGGTGGCAGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1030.1083252702873249No Hit
CCTACGGGCGGCTGCAGTGGTGAAGGCGGATTGCTGGACAGTAACTGACG1030.1083252702873249No Hit
CCTACGGGTGGCTGCAGACGTCGGCAGCGTCAGATGTATGATATCGGGAT1010.10622186698077489No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1000.1051701653274999No Hit
CCTACGGGGGGCAGCAGTGGGGAAGGCGGCTCTCTGGTCTGTAACTGACG1000.1051701653274999No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT990.10411846367422489No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC970.10201506036767491No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT960.1009633587143999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)