FastQCFastQC Report
Wed 8 May 2024
EGAF00008119307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64157
Total Bases19.3 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT63679.92409246068239No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT51868.083295665321009No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT43996.856617360537431No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT42556.63216796296585No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT38836.052340352572595No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT29364.5762738282650375No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT24553.8265504933210717No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT23183.613011830353664No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC5870.9149430303786024No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC5370.8370092117773587No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC4610.7185498075034681No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC4070.6343812834141247No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC4010.6250292251819755No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3180.49565908630391076No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3170.49410040993188586No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2970.46292688249138836No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2660.4146079149586171No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2500.3896690930062191No Hit
ACTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2460.38343438751811965No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2400.37408232928597035No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2380.3709649765419206No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2310.36005424193774643No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2220.34602615458952257No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2010.3132939507770002No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1880.2930311579406768No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1770.27588571784840316No Hit
ACTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1750.2727683651043534No Hit
ACTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1630.2540642486400549No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1600.24938821952398024No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1580.24627086677993046No Hit
ACTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1570.2447121904079056No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1560.24315351403588073No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1550.24159483766385587No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1530.23847748491980608No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1490.2322427794317066No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1450.22600807394360709No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1430.22289072119955736No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1360.2119799865953832No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1340.20886263385133347No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1330.20730395747930855No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1180.18392381189893542No Hit
ACTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1060.1652196954346369No Hit
ACTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1040.16210234269058715No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1010.15742631357451253No Hit
ACTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1000.15586763720248767No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC980.1527502844584379No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT970.15119160808641302No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT950.14807425534236326No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC930.1449569025983135No Hit
CATACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT920.14339822622628864No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT890.138722197110214No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC860.13404616799413938No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC850.13248749162211448No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC850.13248749162211448No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT840.13092881525008962No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT840.13092881525008962No Hit
CATACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT840.13092881525008962No Hit
CATACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT780.12157675701794037No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT770.12001808064591547No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC710.11066602241376625No Hit
CATACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT690.10754866966971649No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT670.10443131692566673No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT650.10131396418161698No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)