FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008119311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121716
Sequences flagged as poor quality0
Sequence length301
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT98518.093430608958558No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT79696.54720825528279No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT70805.816819481415755No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT64675.3131880771632325No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT56844.669887278582931No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT40363.3159157382759865No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT39893.2773012586677184No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT34672.848434059614184No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT31292.5707384403036575No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT30452.5017253278122844No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT29452.419566860560649No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT26822.2034900916888494No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT22921.883072069407473No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC18571.5256827368628612No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT16951.3925860199152125No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC14761.2126589766341318No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT14621.201156791218903No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12181.000690131124914No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC11940.9809720989845214No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC11050.9078510631305662No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC10940.8988136317328863No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8030.6597324920306287No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC7910.6498734759604324No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6960.5718229320713792No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5550.455979493246574No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5480.45022840053895957No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5390.44283413848631237No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4780.3927174734628151No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3930.32288277629892537No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGCGGAAGCCTGAT3810.3130237602287292No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGCGGAAGCCTGAT3560.2924841434158204No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGCGGAAGCCTGAT3260.26783660324032993No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3100.25469124848006836No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGCGGAAGCCTGAT3010.24729698642742123No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2850.2341516316671596No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2790.22922212363206151No Hit
CCTATGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2750.2259357849419961No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGCGGAAGCCTGAT2660.21854152288934897No Hit
CCTATGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2600.21361201485425085No Hit
CCTATGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1940.1593874264681718No Hit
CCTATGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1750.14377731769036117No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1750.14377731769036117No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGCGGAAGCCTGAT1750.14377731769036117No Hit
CCTATGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1730.14213414834532848No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGGAAGCCTGAT1600.1314535476026159No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1560.12816720891255054No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1490.12241611620493607No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGCGGAAGCCTGAT1290.10598442275460908No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1280.10516283808209276No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)