FastQCFastQC Report
Wed 8 May 2024
EGAF00008119322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58792
Total Bases17.6 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG8481.4423731119880256No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG8011.3624302626207647No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7241.2314600625935501No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG6691.1379099197169684No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6081.0341543067083956No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6061.0307524833310655No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5800.9865287794257722No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5370.9133895768131719No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4960.8436521975779018No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4860.8266430806912506No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4630.7875221118519525No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4340.738195672880664No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4100.697373792352701No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4010.682065587154715No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3790.6446455300040822No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3400.5783099741461424No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3390.5766090624574772No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2940.5000680364675466No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1870.3180704857803783No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1830.3112668390257178No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1830.3112668390257178No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1750.2976595455163968No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1620.2755476935637502No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1550.2636413117430943No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1520.2585385766770989No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1490.25343584161110355No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1430.2432303714791128No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1410.23982854810178253No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1400.23812763641311743No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1390.2364267247244523No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1350.2296230779697918No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1350.2296230779697918No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1310.22281943121513131No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1250.2126139610831406No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1220.20751122601714517No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1200.20410940263981495No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1190.2024084909511498No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1190.2024084909511498No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACCNNNG1190.2024084909511498No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1160.19730575588515442No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1160.19730575588515442No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1070.18199755068716833No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1040.17689481562117296No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACCTCAG1030.17519390393250783No Hit
GANTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1030.17519390393250783No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACCTCAG1020.17349299224384268No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC970.1649884338005171No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACCTCAG960.16328752211185196No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG960.16328752211185196No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGTGCATGAG950.1615866104231868No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACCTCAG940.1598856987345217No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG930.1581847870458566No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG930.1581847870458566No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGCACCTCAG930.1581847870458566No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG920.15648387535719147No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGTGCATGAG910.1547829636685263No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGCACCTCAG910.1547829636685263No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG910.1547829636685263No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACCTCAG880.14968022860253094No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACCTNNG880.14968022860253094No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACCTNNG880.14968022860253094No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACCNNNG880.14968022860253094No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACCTCAG870.14797931691386582No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG870.14797931691386582No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGTGCATGAG870.14797931691386582No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACCTCAG860.14627840522520072No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGTGCATGAG860.14627840522520072No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACCTCAG860.14627840522520072No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGTGCANNNG850.1445774935365356No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGTGCANNNG840.14287658184787044No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG830.14117567015920532No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACCTNNG830.14117567015920532No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACCNCAG830.14117567015920532No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG830.14117567015920532No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACCTCAG830.14117567015920532No Hit
GNNTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG820.13947475847054022No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGTGCATGAG820.13947475847054022No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGTGCATGAG820.13947475847054022No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGTGCATNNG820.13947475847054022No Hit
GNNTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG810.1377738467818751No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG810.1377738467818751No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC810.1377738467818751No Hit
GNNTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG810.1377738467818751No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG800.13607293509320995No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGTGCATGAG800.13607293509320995No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG800.13607293509320995No Hit
GANTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG790.13437202340454485No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACCNNNG790.13437202340454485No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACCNNNG770.13097020002721457No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG770.13097020002721457No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGTGCANGAG760.12926928833854945No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACCNCAG740.12586746496121923No Hit
GANTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG740.12586746496121923No Hit
GANTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG740.12586746496121923No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGTGCANNNG730.12416655327255409No Hit
GANTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG720.12246564158388895No Hit
GNNTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG720.12246564158388895No Hit
GANTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG720.12246564158388895No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGTGCATGAG710.12076472989522384No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGTGCATGAG700.11906381820655872No Hit
NNCTNNCGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGTGCATGAG700.11906381820655872No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG700.11906381820655872No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG690.11736290651789358No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG690.11736290651789358No Hit
GANTANTAGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGCACCTCAG680.11566199482922848No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG680.11566199482922848No Hit
GANTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG680.11566199482922848No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG680.11566199482922848No Hit
NNCTNNCGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACCTCAG670.11396108314056334No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG670.11396108314056334No Hit
NNNTNNCCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACCNNNG670.11396108314056334No Hit
NANTANCGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGTGCATGAG660.11226017145189822No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGTGCANNNG660.11226017145189822No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGTGCATNNG660.11226017145189822No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGTGCATNNG660.11226017145189822No Hit
GANTANCGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGCACCTCAG660.11226017145189822No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACCTCAG660.11226017145189822No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACCNCAG660.11226017145189822No Hit
NNCTNNCAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACCTCAG650.11055925976323308No Hit
GANTNNTAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACCNCAG650.11055925976323308No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGTGCATGAG640.10885834807456797No Hit
NNNTNNCGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGTGCATGAG640.10885834807456797No Hit
NANTNNCAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACCTCAG640.10885834807456797No Hit
GANTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG640.10885834807456797No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGTGCATGAG630.10715743638590285No Hit
NANTNNAGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGTGCATGAG630.10715743638590285No Hit
NANTANCGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACCTCAG630.10715743638590285No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGTGCATGAG620.10545652469723772No Hit
NNNTNNTAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGTGCATGAG620.10545652469723772No Hit
NNNTNNAGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACCTCAG620.10545652469723772No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACCTCAG620.10545652469723772No Hit
GNNTANCCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACCTNNG610.10375561300857258No Hit
NNNTNNTAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACCTCAG610.10375561300857258No Hit
GANTANCGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGTGCATGAG610.10375561300857258No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG610.10375561300857258No Hit
NNCTNNTAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACCTCAG600.10205470131990747No Hit
GNNTANTAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGTGCATNNG600.10205470131990747No Hit
GANTANCAGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGCACCTCAG590.10035378963124235No Hit
GANTNNCCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACCNCAG590.10035378963124235No Hit
NNCTNNCCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACCTCAG590.10035378963124235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)