FastQCFastQC Report
Wed 9 Aug 2023
EGAF00008119324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49144
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG16573.371723913397363No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG16523.3615497314015954No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG13742.79586521243692No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG13462.7388897932606215No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG13062.6574963372944813No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG13032.651391828097021No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12312.5048836073579683No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11292.2973302946443104No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10582.1528569103044117No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5271.0723587823538987No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5061.029627217971675No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4350.8851538336317761No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4000.8139345596614032No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3910.7956210320690216No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3870.7874816864724077No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3680.7488197948884909No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3550.7223669216994953No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3540.7203320853003419No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT3500.7121927397037278No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3490.7101579033045744No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3470.7060882305062673No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT3390.6898095393130392No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3360.6837050301155787No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3320.6755656845189647No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3160.6430083021325086No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3060.6226599381409734No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3030.616555428943513No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3010.612485756145206No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2870.5839980465570568No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT2830.5758587009604428No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT2770.5636496825655217No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT2490.5066742633892235No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT2370.48225622659938144No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT2260.4598730262086928No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT2010.40900211622985516No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT1980.4028976070323946No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT940.19127462152042976No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT940.19127462152042976No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT930.18923978512127626No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT920.18720494872212273No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT910.18517011232296923No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT860.1749959303272017No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT800.16278691193228065No Hit
GACTACACGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT790.16075207553312715No Hit
GACTACAGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT770.15668240273482012No Hit
GACTACCCGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT730.1485430571382061No Hit
GACTACAAGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT700.14243854794074556No Hit
GACTACCAGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT640.1302295295458245No Hit
GACTACTGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT620.1261598567475175No Hit
GACTACCGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT600.12209018394921049No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT600.12209018394921049No Hit
GACTACTAGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT590.12005534755005698No Hit
GACTACTCGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT590.12005534755005698No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT590.12005534755005698No Hit
GACTACAAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCT570.11598567475174996No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT570.11598567475174996No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCTCCCGTAGGCTGTCTCT550.11191600195344295No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT540.10988116555428944No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT530.10784632915513592No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT530.10784632915513592No Hit
GACTACACGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT530.10784632915513592No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT530.10784632915513592No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT530.10784632915513592No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT520.10581149275598242No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT520.10581149275598242No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT510.10377665635682891No Hit
GACTACCCGGGTATCTAATCCACATCTGCTGCCCCCCGTAGGCTGTCTCT510.10377665635682891No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCAGCCTCCCGTAGGCTGTCTCT510.10377665635682891No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT500.1017418199576754No Hit
GACTACAGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT500.1017418199576754No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)