FastQCFastQC Report
Wed 8 May 2024
EGAF00008119328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70528
Total Bases21.2 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG12041.7071234119782213No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10761.5256352087114338No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG8351.1839269509981851No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7921.1229582577132486No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6280.8904264972776769No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6000.8507259528130671No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5760.8166969147005444No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5120.7259528130671506No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4970.704684664246824No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4320.6125226860254084No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4050.5742400181488203No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3830.5430467332123412No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3450.48916742286751363No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2790.3955875680580762No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2630.37290154264972775No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2620.37148366606170596No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2520.3573049001814882No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2450.34737976406533577No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2240.3176043557168784No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2180.30909709618874776No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2160.3062613430127042No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2150.3048434664246824No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2100.2977540834845735No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2050.2906647005444646No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1970.2793216878402904No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1930.27365018148820325No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1850.26230716878402904No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1680.2382032667876588No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1680.2382032667876588No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1660.23536751361161526No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1610.22827813067150637No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1540.21835299455535392No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1530.21693511796733211No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1490.21126361161524504No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1480.20984573502722323No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACCTCAG1450.20559210526315788No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACCTNNG1440.2041742286751361No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1410.19992059891107078No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1400.198502722323049No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1400.198502722323049No Hit
GNNTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1360.1928312159709619No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1350.1914133393829401No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACCNNNG1340.18999546279491833No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1320.18715970961887476No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1310.18574183303085298No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACCTCAG1290.18290607985480944No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1270.18007032667876588No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACCTCAG1260.1786524500907441No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACCNCAG1250.17723457350272231No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACCTCAG1240.17581669691470056No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1240.17581669691470056No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1210.1715630671506352No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1210.1715630671506352No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGCACCTCAG1210.1715630671506352No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACCTCAG1200.17014519056261343No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACCTCAG1200.17014519056261343No Hit
GNNTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1160.1644736842105263No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1160.1644736842105263No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGCACCTCAG1150.16305580762250454No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACCTCAG1150.16305580762250454No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1130.16022005444646098No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACCTCAG1100.15596642468239563No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1100.15596642468239563No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1100.15596642468239563No Hit
GANTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1080.1531306715063521No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACCNCAG1080.1531306715063521No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACCTCAG1070.15171279491833028No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1060.15029491833030853No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1040.147459165154265No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1040.147459165154265No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1040.147459165154265No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACCNNNG1030.1460412885662432No Hit
GANTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1020.1446234119782214No Hit
NANTANTAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACCTCAG970.13753402903811252No Hit
GNNTANTAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACCTNNG940.1332803992740472No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG940.1332803992740472No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG940.1332803992740472No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA920.13044464609800363No Hit
NNNTNNCCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACCTCAG920.13044464609800363No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG920.13044464609800363No Hit
NNNTNNTAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACCTCAG920.13044464609800363No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG920.13044464609800363No Hit
GANTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG910.12902676950998185No Hit
NNNTNNCCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACCNNNG900.12760889292196007No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG900.12760889292196007No Hit
GANTNNCCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACCNCAG890.1261910163339383No Hit
NNCTNNTAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACCTCAG880.12477313974591651No Hit
NNNTNNCGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACCTCAG880.12477313974591651No Hit
NANTNNCAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACCTCAG870.12335526315789473No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACCNCAG870.12335526315789473No Hit
NNCTNNCGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACCTCAG860.12193738656987296No Hit
GANTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG860.12193738656987296No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG860.12193738656987296No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACCNNNG850.12051950998185118No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACCNNNG850.12051950998185118No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG850.12051950998185118No Hit
GANTNNTAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACCNCAG850.12051950998185118No Hit
GANTANCGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGCACCTCAG840.1191016333938294No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG840.1191016333938294No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACCTNNG830.11768375680580763No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA830.11768375680580763No Hit
GANTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG830.11768375680580763No Hit
GANTANCCGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGCACCTCAG810.11484800362976405No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACCTCAG810.11484800362976405No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACCTNNG800.1134301270417423No Hit
NANTANCAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACCTCAG790.11201225045372051No Hit
GANTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG790.11201225045372051No Hit
NNNTNNCAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACCNNNG780.11059437386569872No Hit
NANTNNAGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACCTCAG780.11059437386569872No Hit
NANTANCGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACCTCAG770.10917649727767696No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG770.10917649727767696No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG760.10775862068965517No Hit
GANTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG760.10775862068965517No Hit
NNNTNNCAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACCTCAG760.10775862068965517No Hit
GANTANTAGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGCACCTCAG750.10634074410163338No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACCTCAG750.10634074410163338No Hit
NNCTNNACGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACCTCAG740.10492286751361161No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG740.10492286751361161No Hit
NNCTNNCCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACCTCAG740.10492286751361161No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG740.10492286751361161No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG720.10208711433756805No Hit
GANTANCAGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGCACCTCAG720.10208711433756805No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG710.10066923774954628No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG710.10066923774954628No Hit
NNCTNNAGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACCTCAG710.10066923774954628No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)