FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008119358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85808
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9921.1560693641618496No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9311.0849804214059295No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7400.8623904531046056No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6800.7924669028528808No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6400.7458512026850643No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5940.6922431474920754No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4860.5663807570389707No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4800.5593884020137982No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4330.5046149543166139No Hit
GNNTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2880.33563304120827897No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2830.3298060786873019No Hit
GNNTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2200.2563863509229909No Hit
GNNTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2090.24356703337684132No Hit
GNNTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1950.22725153831810557No Hit
GNNTANCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1810.21093604325936977No Hit
NNNTNNTCGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC1690.1969513332090248No Hit
NNNTNNTCGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC1630.18995897818385232No Hit
NANTNNTCGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC1600.18646280067126608No Hit
GNNTANTGGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC1590.18529740816707066No Hit
NANTNNTGGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC1570.18296662315867984No Hit
GANTNNTCGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC1470.17131269811672573No Hit
GNNTANAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1420.16548573559574864No Hit
GANTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1410.16432034309155324No Hit
GNNTANACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1410.16432034309155324No Hit
GANTANTCGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC1400.16315495058735782No Hit
NNCTNNTGGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC1400.16315495058735782No Hit
NNNTNNTGGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC1390.1619895580831624No Hit
GNNTANTCGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC1360.15849338057057616No Hit
GANTNNTGGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC1350.15732798806638076No Hit
GANTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1300.15150102554540368No Hit
GNNTANAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1290.15033563304120828No Hit
NNNTNNTGGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC1280.14917024053701286No Hit
GANTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1260.14683945552862204No Hit
NANTANTCGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC1250.14567406302442662No Hit
NANTANCGGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC1250.14567406302442662No Hit
NANTANTGGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC1240.1445086705202312No Hit
NNCTNNTCGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC1160.1351855304866679No Hit
NANTNNCAGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC1130.13168935297408169No Hit
GNNTANTAGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC1120.13052396046988626No Hit
NANTNNTAGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC1120.13052396046988626No Hit
GANTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1110.12935856796569084No Hit
NNCTNNTAGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC1090.12702778295730002No Hit
NNNTNNTAGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC1090.12702778295730002No Hit
GANTANTGGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC1090.12702778295730002No Hit
GANTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1080.12586239045310463No Hit
NNNTNNTAGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC1050.12236621294051837No Hit
GANTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1040.12120082043632295No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1030.12003542793212754No Hit
NANTANTAGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC1030.12003542793212754No Hit
GANTNNTAGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC1030.12003542793212754No Hit
NNNTNNCCGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC1020.11887003542793213No Hit
NNNTNNCCGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC1010.11770464292373672No Hit
GANTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1000.1165392504195413No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG980.11420846541115048No Hit
GNNTANCGGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC970.11304307290695506No Hit
NNNTNNCGGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC970.11304307290695506No Hit
NANTNNCCGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC970.11304307290695506No Hit
GNNTANCCGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC960.11187768040275967No Hit
NNNTNNCAGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC960.11187768040275967No Hit
GANTANTAGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC960.11187768040275967No Hit
GANTANCCGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC950.11071228789856424No Hit
NANTNNCGGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC950.11071228789856424No Hit
NNNTNNAGGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC920.107216110385978No Hit
NANTNNAGGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC920.107216110385978No Hit
NNCTNNCGGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC910.10605071788178258No Hit
NANTANCCGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC910.10605071788178258No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC910.10605071788178258No Hit
GANTNNCGGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC910.10605071788178258No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA900.10488532537758716No Hit
GANTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC890.10371993287339176No Hit
NNNTNNCAGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC890.10371993287339176No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA880.10255454036919634No Hit
NNNTNNCGGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC880.10255454036919634No Hit
NNCTNNCAGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC860.10022375536080552No Hit
GANTNNCAGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC860.10022375536080552No Hit
GNNTANCAGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC860.10022375536080552No Hit
GANTNNCCGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC860.10022375536080552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)