FastQCFastQC Report
Wed 9 Aug 2023
EGAF00008119363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81148
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC865910.67062650958742No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC68908.490659042736729No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC64047.891753339577069No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC58927.2608074136146294No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC50646.240449548972248No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC40044.934194311628136No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC32163.96312909745157No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC29683.657514664563514No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT14591.7979494257406219No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT11851.4602947700497855No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT11361.3999112732291616No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT9821.2101345689357716No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT9341.150983388376793No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7090.8737122295065805No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5350.6592891999802829No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4920.6062996007295313No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2940.36230098092374424No Hit
CCTACGGGGAGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2010.24769556859072311No Hit
CCTACGGGTAGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2010.24769556859072311No Hit
CCTACGGGTAGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1970.24276630354414155No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1960.2415339872824962No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1940.23906935475920538No Hit
CATACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1920.23660472223591464No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1870.23044314092768767No Hit
CCTACGGGGAGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1830.2255138758811061No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1780.2193522945728792No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1440.17745354167693597No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1360.16759501158377285No Hit
CATACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1330.1638980627988367No Hit
CATACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1330.1638980627988367No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1240.1528072164440282No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1220.15034258392073743No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1160.14294868635086508No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1160.14294868635086508No Hit
CCTACGGGCAGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1150.1417163700892197No Hit
CCTACGGGCAGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1120.13801942130428352No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1110.13678710504263814No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1110.13678710504263814No Hit
CCTACGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGACG1100.13555478878099275No Hit
ACTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1060.1306255237344112No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1050.1293932074727658No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1020.12569625868782966No Hit
CATACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1000.12323162616453888No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT950.11707004485631192No Hit
CATACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC950.11707004485631192No Hit
CCTACGGGAAGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC940.11583772859466654No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT920.11337309607137576No Hit
CTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGACG890.1096761472864396No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT860.10597919850150342No Hit
CCTACGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGACG830.10228224971656726No Hit
CATACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC830.10228224971656726No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)