FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008119367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42447
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT17744.179329516809197No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11292.659787499705515No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11022.5961787641058263No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10282.4218437109807525No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10102.3794378872476263No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9322.195679317737414No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7891.8587886069686903No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7761.8281621787169882No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6481.5266096543925367No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6431.5148302589111127No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4841.140245482601833No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4521.06485735152072No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4401.0365868023653027No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4321.0177397695950243No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4271.0059603741136005No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC4140.9753339458618983No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3700.8716752656253681No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3650.8598958701439442No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3610.8504723537588051No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3520.829269441892242No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3410.8033547718331094No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3370.7939312554479704No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3270.7703724644851226No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCGTGGG2730.6431549932857445No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2660.6266638396117511No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2390.5630551040120622No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2340.5512757085306382No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2260.53242867576036No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2250.5300727966640751No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2230.5253610384715056No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2070.4876669729309491No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1840.43348175371639924No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCGTGGG1730.4075670836572667No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1700.4004994463684124No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCGTGGG1630.38400829269441894No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCGTGGG1620.3816524135981342No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCGTGGG1570.36987301811671025No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1490.351025985346432No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCGTGGG1490.351025985346432No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCGTGGG1440.3392465898650081No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1410.3321789525761538No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1400.32982307347986906No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1390.32746719438358424No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1360.3203995570947299No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1340.31568779890216037No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCGTGGG1250.29448488703559733No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1020.24029966782104745No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1000.23558790962847787No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC940.2214526350507692No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT920.21674087685819962No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCTGGTAGTCCTGTCTCT890.20967323956934528No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT880.2073173604730605No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCCGGTAGTCCTGTCTCT870.20496148137677575No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT870.20496148137677575No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT870.20496148137677575No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCTTGTAGTCCTGTCTCT860.20260560228049096No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT840.1978938440879214No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCGGGTAGTCCTGTCTCT840.1978938440879214No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT790.1861144486064975No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCGAGTAGTCCTGTCTCT760.17904681131764316No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCCAGTAGTCCTGTCTCT730.17197917402878884No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT720.16962329493250405No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC650.1531321412585106No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC650.1531321412585106No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT630.14842038306594105No Hit
CCTACGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATG620.14606450396965628No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT610.1437086248733715No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT600.1413527457770867No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT590.13899686668080194No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT590.13899686668080194No Hit
CCTACGGGCGGCTGCAGATGTGGATTAGATACCCTTGTAGTCCTGTCTCT580.13664098758451718No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCTAGTAGTCCTGTCTCT580.13664098758451718No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT550.12957335029566283No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCCTGTAGTCCTGTCTCT530.12486159210309328No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC530.12486159210309328No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT530.12486159210309328No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCGTGTAGTCCTGTCTCT520.12250571300680849No Hit
CCTACGGGGGGCTGCAGATGTGGATTAGATACCCTTGTAGTCCTGTCTCT520.12250571300680849No Hit
CCTACGGGGGGCTGCAGATGTGGATTAGATACCCGGGTAGTCCTGTCTCT520.12250571300680849No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC520.12250571300680849No Hit
CCTACGGGTGGCTGCAGATGTGGATTAGATACCCTTGTAGTCCTGTCTCT510.12014983391052372No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT510.12014983391052372No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT510.12014983391052372No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC510.12014983391052372No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT510.12014983391052372No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT500.11779395481423893No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT500.11779395481423893No Hit
CCTACGGGTGGCTGCAGATGTGGATTAGATACCCCAGTAGTCCTGTCTCT500.11779395481423893No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT500.11779395481423893No Hit
CCTACGGGTGGCAGCAGATGTGGATTAGATACCCCAGTAGTCCTGTCTCT500.11779395481423893No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT500.11779395481423893No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT490.11543807571795416No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC490.11543807571795416No Hit
CCTACGGGTGGCAGCAGATGTGGATTAGATACCCTTGTAGTCCTGTCTCT490.11543807571795416No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT490.11543807571795416No Hit
CCTACGGGCGGCTGCAGATGTGGATTAGATACCCGGGTAGTCCTGTCTCT490.11543807571795416No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT480.11308219662166938No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT470.1107263175253846No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT470.1107263175253846No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT470.1107263175253846No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT470.1107263175253846No Hit
CCTACGGGGGGCAGCAGATGTGGATTAGATACCCTGGTAGTCCTGTCTCT460.10837043842909981No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT460.10837043842909981No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT460.10837043842909981No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT450.10601455933281505No Hit
CCTACGGGCGGCAGCAGATGTGGATTAGATACCCCAGTAGTCCTGTCTCT450.10601455933281505No Hit
CCTACGGGAGGCTGCAGATGTGGATTAGATACCCCAGTAGTCCTGTCTCT440.10365868023653026No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT440.10365868023653026No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT440.10365868023653026No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT440.10365868023653026No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT440.10365868023653026No Hit
CCTACGGGGGGCTGCAGATGTGGATTAGATACCCCGGTAGTCCTGTCTCT440.10365868023653026No Hit
CCTACGGGAGGCTGCAGATGTGGATTAGATACCCTGGTAGTCCTGTCTCT440.10365868023653026No Hit
CCTACGGGGGGCAGCAGATGTGGATTAGATACCCGAGTAGTCCTGTCTCT440.10365868023653026No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT440.10365868023653026No Hit
CCTACGGGCGGCTGCAGATGTGGATTAGATACCCCGGTAGTCCTGTCTCT440.10365868023653026No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT430.10130280114024548No Hit
CCTACGGGCGGCAGCAGATGTGGATTAGATACCCGGGTAGTCCTGTCTCT430.10130280114024548No Hit
CCTACGGGAGGCTGCAGATGTGGATTAGATACCCTTGTAGTCCTGTCTCT430.10130280114024548No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT430.10130280114024548No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT430.10130280114024548No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)