FastQCFastQC Report
Wed 8 May 2024
EGAF00008119368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42447
Total Bases12.7 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG13233.116828044384762No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12012.829410794638019No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10432.4571818974250244No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10152.39121728272905No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10122.3841496454401963No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9002.1202911866563006No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8912.0990882747897377No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8241.9412443753386577No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7851.849365090583551No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5251.2368365255495088No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5061.1920748227200981No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5001.1779395481423893No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4551.0719249888095743No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4471.053077956039296No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4421.0412985605578722No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4291.01067213230617No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4261.0036044950173157No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3940.9282163639362028No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3840.904657572973355No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3650.8598958701439442No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3340.7868636181591161No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3280.7727283435814074No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3230.7609489480999835No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3220.7585930690036987No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3180.7491695526185597No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3130.7373901571371357No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3080.7256107616557118No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2660.6266638396117511No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2600.6125285650340424No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2580.6078168068414729No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2570.6054609277451881No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2380.5606992249157773No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2310.5442080712417838No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2280.5371404339529295No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2220.5230051593752209No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCCCATTGT1810.4264141164275449No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1780.41934647913869055No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCCCATTGT1640.3863641717907037No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCCCATTGT1610.37929653450184936No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCCCATTGT1600.3769406554055646No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCCCATTGT1590.37458477630927983No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCCCATTGT1580.372228897212995No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCCCATTGT1330.31333191980587555No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCCCATTGT1210.2850613706504582No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCCCATTGT1180.2779937333616039No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT1050.24736730510990174No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT950.22380851414705397No Hit
GACTACCAGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT920.21674087685819962No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT910.21438499776191486No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT870.20496148137677575No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT840.1978938440879214No Hit
GACTACCGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT820.19318208589535185No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT800.1884703277027823No Hit
GACTACTGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT760.17904681131764316No Hit
GACTACAAGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT740.1743350531250736No Hit
GACTACCCGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT730.17197917402878884No Hit
GACTACTCGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT720.16962329493250405No Hit
GACTACACGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT690.16255565764364974No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT660.1554880203547954No Hit
GACTACTAGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT600.1413527457770867No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT590.13899686668080194No Hit
GACTACAGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT560.13192922939194762No Hit
GACTACAAGGGTATCTAATCCACATCTGCAGCCGCCCGTAGGCTGTCTCT520.12250571300680849No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA520.12250571300680849No Hit
GACTACTGGGGTATCTAATCCACATCTGCAGCCACCCGTAGGCTGTCTCT510.12014983391052372No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT510.12014983391052372No Hit
GACTACCCGGGTATCTAATCCACATCTGCAGCCGCCCGTAGGCTGTCTCT510.12014983391052372No Hit
GACTACACGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT510.12014983391052372No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT500.11779395481423893No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT500.11779395481423893No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT500.11779395481423893No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA500.11779395481423893No Hit
GACTACAGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT500.11779395481423893No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT490.11543807571795416No Hit
GACTACTGGGGTATCTAATCCACATCTGCTGCCGCCCGTAGGCTGTCTCT490.11543807571795416No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT490.11543807571795416No Hit
GACTACAAGGGTATCTAATCCACATCTGCAGCCCCCCGTAGGCTGTCTCT480.11308219662166938No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT480.11308219662166938No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT470.1107263175253846No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT470.1107263175253846No Hit
GACTACTGGGGTATCTAATCCACATCTGCAGCCTCCCGTAGGCTGTCTCT460.10837043842909981No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT460.10837043842909981No Hit
GACTACTGGGGTATCTAATCCACATCTGCTGCCACCCGTAGGCTGTCTCT460.10837043842909981No Hit
GACTACTCGGGTATCTAATCCACATCTGCTGCCCCCCGTAGGCTGTCTCT450.10601455933281505No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT450.10601455933281505No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT450.10601455933281505No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT450.10601455933281505No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT450.10601455933281505No Hit
GACTACCGGGGTATCTAATCCACATCTGCAGCCGCCCGTAGGCTGTCTCT440.10365868023653026No Hit
GACTACCCGGGTATCTAATCCACATCTGCAGCCCCCCGTAGGCTGTCTCT440.10365868023653026No Hit
GACTACAGGGGTATCTAATCCACATCTGCAGCCACCCGTAGGCTGTCTCT440.10365868023653026No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT440.10365868023653026No Hit
GACTACAAGGGTATCTAATCCACATCTGCTGCCACCCGTAGGCTGTCTCT430.10130280114024548No Hit
GACTACCGGGGTATCTAATCCACATCTGCAGCCCCCCGTAGGCTGTCTCT430.10130280114024548No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT430.10130280114024548No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA430.10130280114024548No Hit
GACTACAAGGGTATCTAATCCACATCTGCAGCCACCCGTAGGCTGTCTCT430.10130280114024548No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)