FastQCFastQC Report
Wed 8 May 2024
EGAF00008119370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44240
Total Bases13.3 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3220.7278481012658228No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3190.7210669077757685No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2400.5424954792043399No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2030.4588607594936709No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2000.45207956600361665No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1820.41139240506329117No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1770.40009041591320077No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1720.3887884267631103No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1650.3729656419529837No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1380.31193490054249545No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1310.2961121157323689No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1300.2938517179023508No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1200.27124773960216997No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1170.26446654611211573No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1140.2576853526220615No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1090.24638336347197107No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1070.2418625678119349No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1060.23960216998191683No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1000.22603978300180833No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC950.2147377938517179No Hit
GNNTANCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA940.21247739602169982No Hit
GNNTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA920.20795660036166366No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA910.20569620253164558No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA860.19439421338155516No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTANCCNCACNTTCGAGCCTCAA840.18987341772151897No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC840.18987341772151897No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC790.17857142857142858No Hit
GNNTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA780.1763110307414105No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA750.16952983725135623No Hit
GNNTANTCGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC740.16726943942133815No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTANCCNNACTTTCGAGCCNCAA720.162748643761302No Hit
GNNTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC720.162748643761302No Hit
GNNTANCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA710.1604882459312839No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTANCCNCACTTTCGAGCCTCAA690.15596745027124773No Hit
GNNTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA670.15144665461121157No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG670.15144665461121157No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC670.15144665461121157No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTANCCNCANTTTCGAGCCNNNA670.15144665461121157No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTANCCNCANTTTCGAGCCNNNA670.15144665461121157No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTANCCNCACNTTCGAGCCTCAA660.1491862567811935No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTANCNNCANTTTCGAGCCTNNA650.1469258589511754No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTANCCNCACTTTCGAGCCTCAA650.1469258589511754No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTANCCNNANTTTCGAGCCTCAA650.1469258589511754No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTANCNNCANTTTCGAGCCTNNA650.1469258589511754No Hit
GNNTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC640.14466546112115733No Hit
NANTNNTGGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC640.14466546112115733No Hit
GANTNNTAGGGTATNTAATNNTGTTTGNTANCCNNACTTTCGAGCCNCAA630.14240506329113925No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTANCCNNACTTTCGAGCCNCAA630.14240506329113925No Hit
NANTNNTCGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC620.14014466546112117No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC620.14014466546112117No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTANCCNCANTTTCGAGCCTCAA600.13562386980108498No Hit
GANTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA600.13562386980108498No Hit
NNCTNNTCGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC580.13110307414104883No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGATACCCACANTTTCGAGCCTCAA580.13110307414104883No Hit
NANTANTCGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC580.13110307414104883No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG570.12884267631103075No Hit
GANTANTGGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC570.12884267631103075No Hit
GNNTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC560.12658227848101267No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTANCCNCACNTTCGAGCCTCAA560.12658227848101267No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGATACCCACANTTTCGAGCCTCAA560.12658227848101267No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG550.12432188065099457No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTANCCNNANTTTCGAGCCTCAA550.12432188065099457No Hit
GANTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA550.12432188065099457No Hit
NNNTNNTCGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC550.12432188065099457No Hit
GANTANCGGGGTNTCTNNTNNTGTTTGATACCCACANTTTCGAGCCTCAA540.1220614828209765No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG540.1220614828209765No Hit
GNNTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA540.1220614828209765No Hit
GNNTANCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC530.11980108499095841No Hit
GNNTANAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC530.11980108499095841No Hit
GNNTANACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC530.11980108499095841No Hit
GANTANTCGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC530.11980108499095841No Hit
NNNTNNCGGGGTATNTNNTNNTGTTTGNTANCCNCANTTTCGAGCCTCAA530.11980108499095841No Hit
NNNTNNTAGGGTATNTNNTNNTGTTTGNTANCCNCANTTTCGAGCCTCAA520.11754068716094034No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTANCCNNACTTTCGAGCCNCAA520.11754068716094034No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTANCCNCANTTTCGAGCCTCAA520.11754068716094034No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTANCCNCACNTTCGAGCCTCAA500.11301989150090416No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTANCCNCANTTTCGAGCCNNNA500.11301989150090416No Hit
GNNTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC500.11301989150090416No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC500.11301989150090416No Hit
NNCTNNCGGGGTATNTANTCNTGTTTGNTANCCNCACTTTCGAGCCTCAA490.11075949367088608No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTANCCNCACNTTCGAGCCTCAA490.11075949367088608No Hit
GANTANCCGGGTNTCTNNTNNTGTTTGATACCCACANTTTCGAGCCTCAA490.11075949367088608No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG490.11075949367088608No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA490.11075949367088608No Hit
GANTANTAGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC490.11075949367088608No Hit
GACTACTCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA480.108499095840868No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG480.108499095840868No Hit
NANTNNAGGGGTNTNTNNTCNTGTNTGNTANCCNCACNTTCGAGCCTCAA480.108499095840868No Hit
GNNTANTAGGGTNTNTNNTNNTGTTTGNTANCNNCANTTTCGAGCCTNNA480.108499095840868No Hit
NNNTNNCCGGNNNTNTNATNNTGTTTGNTANCCNCANTTTCGAGCCNNNA470.10623869801084991No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC470.10623869801084991No Hit
GNNTANCCGGGTNTNTNNTNNTGTTTGNTANCNNCANTTTCGAGCCTNNA470.10623869801084991No Hit
GANTNNCGGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC460.10397830018083183No Hit
NNNTNNAGGGGTATNTNNTNNTGTTTGNTANCCNCANTTTCGAGCCTCAA460.10397830018083183No Hit
NNNTNNTAGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC460.10397830018083183No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA460.10397830018083183No Hit
NNNTNNTGGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC450.10171790235081375No Hit
GNNTANTGGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC450.10171790235081375No Hit
NNCTNNCCGGGTATNTANTCNTGTTTGNTANCCNCACTTTCGAGCCTCAA450.10171790235081375No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA450.10171790235081375No Hit
NNCTNNTGGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC450.10171790235081375No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC450.10171790235081375No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)