FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008119371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80322
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC17602.1911804984935634No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT16782.089091407086477No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC13101.6309354846741866No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12681.5786459500510446No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12481.5537461716590724No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11351.4130624237444287No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC11201.3943875899504494No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10321.2848285650257714No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC10101.2574388087946018No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC10071.253703842035806No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT10071.253703842035806No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9451.1765145290206918No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC9031.1242249943975497No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8081.0059510470356814No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7840.9760713129653147No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7780.968601379447723No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC7750.9648664126889271No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7700.9586414680909341No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6950.8652672991210377No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6790.84534747640746No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6680.8316525982918752No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6410.7980378974627125No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6250.7781180747491347No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6030.7507283185179652No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5870.7308084958043873No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5830.7258285401259928No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5810.7233385622867956No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5760.7171136176888026No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5730.7133786509300067No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5570.6934588282164289No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5560.6922138392968302No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4650.5789198476133562No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4510.5614900027389756No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4380.5453051467841936No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4170.5191603794726227No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4130.5141804237942282No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3880.48305570080426286No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3650.4544209556534947No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3350.4170712880655362No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3040.3784766315579791No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3020.3759866537187819No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3000.3734966758795847No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2710.3373919972112248No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2420.30128731854286495No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2350.29257239610567465No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2310.2875924404272802No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2300.2863474515076816No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2000.24899778391972313No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1870.23281292796494113No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1770.22036303876895497No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1760.21911804984935634No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1730.2153830830905605No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1690.21040312741216605No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1610.20044321605537713No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1560.19421827145738405No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1520.18923831577898956No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1490.18550334902019372No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1450.18052339334179926No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1420.17678842658300342No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1400.17429844874380618No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1360.16931849306541172No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1320.16433853738701726No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1310.16309354846741864No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1270.15811359278902418No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1260.15686860386942555No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1090.1357037922362491No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1060.13196882547745326No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1060.13196882547745326No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1030.1282338587186574No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT980.12200891412066434No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT930.11578396952267125No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC920.11453898060307262No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT910.11329399168347402No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT900.11204900276387539No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT890.1108040138442768No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT890.1108040138442768No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC880.10955902492467817No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT880.10955902492467817No Hit
CCTACGGGTGGCTGCAGTGGCGAAGGCGGATTGCTGGACGACAACTGACG870.10831403600507956No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT860.10706904708548094No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC850.10582405816588233No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC840.10457906924628371No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC830.10333408032668508No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT820.10208909140708648No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT810.10084410248748785No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG810.10084410248748785No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)