FastQCFastQC Report
Wed 8 May 2024
EGAF00008119395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63385
Total Bases19 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC30224.767689516447109No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC18902.9817780231916067No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC17422.748284294391418No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT17152.70568746548868No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC16802.650469353948095No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC16632.623649128342668No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC15892.506902263942573No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC12712.0052062790881124No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11661.8395519444663566No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10451.6486550445689043No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10321.6281454602824015No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC10231.613946517314822No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10151.601325234676974No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT9321.4703794273093003No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9251.4593358050011833No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT9161.4451368620336043No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7521.1864005679577188No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6521.0286345349846178No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT6411.0112802713575766No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6180.9749940837737635No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT5970.9418632168494123No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5900.9308195945412953No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT5810.9166206515737161No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT5730.9039993689358681No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5710.9008440482764061No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5120.8077620888222765No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT5100.8046067681628145No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4630.7304567326654571No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4600.7257237516762641No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4600.7257237516762641No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT4570.7209907706870711No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC4070.6421077542005206No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4050.6389524335410586No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3970.6263311509032106No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3300.5206279088112329No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3230.5095842865031158No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3180.5016959848544609No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2960.46698745760037863No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2890.45594383529226157No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2820.44490021298414456No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2790.44016723199495145No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2750.43385659067602744No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2710.42754594935710344No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2620.4133470063895244No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2600.41019168573006237No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2590.4086140254003313No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2560.40388104441113826No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2540.40072572375167625No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2490.39283742210302125No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2480.39125976177329025No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2480.39125976177329025No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2480.39125976177329025No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2390.37706081880571113No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2370.3739054981462491No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2350.3707501774867871No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2130.3360416502327049No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2090.3297310089137809No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2060.3249980279245879No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1890.2981778023191607No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1860.2934448213299677No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1830.28871184034077463No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1820.2871341800110436No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1770.27924587836238857No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1700.2682022560542715No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1680.2650469353948095No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1650.26031395440561644No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1640.25873629407588544No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1600.25242565275696144No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1420.22402776682180328No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1380.21771712550287922No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1280.20194052220556913No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1260.19878520154610713No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1180.18616391890825906No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT770.12147984538928769No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT730.11516920407036366No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT720.11359154374063264No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT710.11201388341090164No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT700.11043622308117064No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT700.11043622308117064No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)