FastQCFastQC Report
Tue 7 May 2024
EGAF00008119406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15874
Total Bases4.7 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7714.856998866070303No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7614.794002771828146No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6814.290034017890891No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6704.220738314224518No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6594.151442610558146No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6023.7923648733778506No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5783.641174247196674No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5083.200201587501575No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4953.1183066649867706No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1621.020536726722943No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1621.020536726722943No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1601.0079375078745119No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1520.9575406324807862No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1500.9449414136323547No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1500.9449414136323547No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1430.9008441476628449No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1410.8882449288144136No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1380.8693461005417664No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1370.8630464911175507No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1360.8567468816933351No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1320.8315484439964722No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1280.8063500062996094No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1250.7874511780269624No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1230.774851959178531No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1140.7181554743605896No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1120.7055562555121583No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1090.6866574272395111No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1080.6803578178152955No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1080.6803578178152955No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG910.5732644576036285No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG900.566964848179413No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG870.5480660199067658No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG840.5291671916341186No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG800.5039687539372559No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG760.4787703162403931No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG710.4472722691193146No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC580.36537734660451054No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC500.31498047121078493No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC450.2834824240897065No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC420.2645835958170593No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC420.2645835958170593No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC410.25828398639284367No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC390.24568476754441224No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG350.22048632984754943No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG340.21418672042333378No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG340.21418672042333378No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC300.18898828272647095No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG260.16378984502960817No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG250.15749023560539246No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC250.15749023560539246No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA250.15749023560539246No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG250.15749023560539246No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG250.15749023560539246No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG240.15119062618117676No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG230.14489101675696106No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG230.14489101675696106No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG220.1385914073327454No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG220.1385914073327454No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG210.13229179790852966No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG210.13229179790852966No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA200.12599218848431398No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG200.12599218848431398No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG200.12599218848431398No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG200.12599218848431398No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG200.12599218848431398No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG200.12599218848431398No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG200.12599218848431398No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG190.11969257906009828No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA190.11969257906009828No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG180.11339296963588258No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG180.11339296963588258No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG180.11339296963588258No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG170.10709336021166689No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG170.10709336021166689No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG170.10709336021166689No Hit
GACTACCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAGC170.10709336021166689No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG170.10709336021166689No Hit
GACTACGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAGC160.10079375078745118No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA160.10079375078745118No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG160.10079375078745118No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG160.10079375078745118No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG160.10079375078745118No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG160.10079375078745118No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG160.10079375078745118No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)