FastQCFastQC Report
Wed 8 May 2024
EGAF00008119414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199559
Total Bases60 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG101105.066170906849603No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG100235.022574777384132No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG84874.25287759509719No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG84304.2243146137232594No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG83264.172199700339248No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG78853.9512124233935833No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG75233.769812436422311No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG70303.5227677027846402No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG69553.4851848325557855No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG17670.885452422591815No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG17510.8774347436096593No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG14130.7080612751116211No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13960.6995424911930808No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13640.6835071332287694No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13460.6744872443738443No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12400.6213701211170631No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11580.5802795163335154No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG11420.5722618373513597No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11110.5567275843234332No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG10880.5452021707865845No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9410.4715397451380294No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9220.46201875134671955No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9040.45299886249179444No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG8800.44097234401856095No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG8030.4023872639169369No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG7990.40038284417139797No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG7550.3783342269704699No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7260.36380218381531276No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6970.34927014066015566No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG6770.3392480419324611No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG6380.3197049494134567No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG6240.3126894803040705No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG6210.3111861654949163No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5980.2996607519580675No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5860.2936474927214508No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5590.28011765943906314No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5330.2670889310930602No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4880.2445392089557474No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4770.23902705465551544No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4690.2350182151644376No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4660.2335149003552834No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4590.2300071658005903No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4570.22900495592782086No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4430.22198948681843464No Hit
GACTACTGGGGTATCTAATCCTCCCAGTTGCTACAGAGACAAAGTGGTCT4410.2209872769456652No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4240.2124684930271248No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4140.2074574436632775No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4130.20695633872689279No Hit
GACTACCGGGGTATCTAATCCTCCCAGTTGCTACAGAGACAAAGTGGTCT4120.20645523379050806No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG4050.20294749923581495No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4010.20094307949027607No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3990.19994086961750662No Hit
GACTACAAGGGTATCTAATCCTCCCAGTTGCTACAGAGACAAAGTGGTCT3970.19893865974473715No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3900.19543092519004407No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3870.19392761038088985No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG3840.19242429557173568No Hit
GACTACCCGGGTATCTAATCCTCCCAGTTGCTACAGAGACAAAGTGGTCT3820.19142208569896624No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3810.1909209807625815No Hit
GACTACTCGGGTATCTAATCCTCCCAGTTGCTACAGAGACAAAGTGGTCT3780.18941766595342732No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3760.18841545608065785No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3680.18440661658958002No Hit
GACTACCGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG3680.18440661658958002No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3620.18139998697127166No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG3560.17839335735296327No Hit
GACTACAGGGGTATCTAATCCTCCCAGTTGCTACAGAGACAAAGTGGTCT3550.17789225241657855No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG3540.17739114748019383No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG3530.17689004254380908No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG3520.17638893760742436No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3510.17588783267103963No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3490.1748856227982702No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG3450.17288120305273127No Hit
GACTACCAGGGTATCTAATCCTCCCAGTTGCTACAGAGACAAAGTGGTCT3450.17288120305273127No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3430.17187899317996183No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3420.17137788824357708No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3400.17037567837080764No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3380.16937346849803817No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3380.16937346849803817No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3320.1663668388797298No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3250.1628591043250367No Hit
GACTACACGGGTATCTAATCCTCCCAGTTGCTACAGAGACAAAGTGGTCT3210.16085468457949778No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3200.16035357964311306No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3200.16035357964311306No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3190.15985247470672834No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3150.15784805496118942No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3090.15484142534288106No Hit
GACTACAAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG3080.15434032040649634No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3060.15333811053372687No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3030.1518347957245727No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2970.14882816610626431No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2970.14882816610626431No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2960.1483270611698796No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2910.14582153648795593No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2900.1453204315515712No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2850.14281490686964757No Hit
GACTACTAGGGTATCTAATCCTCCCAGTTGCTACAGAGACAAAGTGGTCT2850.14281490686964757No Hit
GACTACAGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG2830.1418126969968781No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2800.14030938218772393No Hit
GACTACCCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG2780.13930717231495446No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT2700.13529833282387665No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT2660.13329391307833774No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT2650.13279280814195302No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2640.1322917032055683No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2640.1322917032055683No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2640.1322917032055683No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2640.1322917032055683No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2630.13179059826918355No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2590.12978617852364463No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2580.1292850735872599No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2570.12878396865087519No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT2560.12828286371449046No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2560.12828286371449046No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2530.12677954890533627No Hit
GACTACCAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG2500.12527623409618208No Hit
GACTACTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG2490.12477512915979735No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2440.1222696044778737No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2400.12026518473233479No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2340.11725855511402644No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT2310.11575524030487225No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT2310.11575524030487225No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2290.11475303043210279No Hit
GACTACACGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG2270.11375082055933333No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2260.11324971562294861No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT2240.11224750575017915No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2210.11074419094102496No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT2200.11024308600464024No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2190.1097419810682555No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG2120.1062342465135624No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2120.1062342465135624No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2110.10573314157717767No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2090.10473093170440821No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT2080.10422982676802349No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2070.10372872183163875No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2070.10372872183163875No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2030.10172430208609984No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2010.10072209221333041No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2010.10072209221333041No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2000.10022098727694567No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)