FastQCFastQC Report
Wed 9 Aug 2023
EGAF00008119439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68706
Sequences flagged as poor quality0
Sequence length301
%GC55

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT65169.483887870055018No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT44636.4957936715861795No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT41316.01257532093267No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC40005.821907839198905No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT37445.449305737490175No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT37085.396908566937386No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT35265.132011760253835No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT29864.346054201961983No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC27293.9719966232934536No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC25033.643058830378715No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC23023.3505079614589697No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT21833.1773062032428023No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC21533.133641894448811No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC21403.1147206939714143No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC15972.3243967048001632No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC13001.8921200477396445No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1880.27362966844234854No Hit
CATACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1330.1935784356533636No Hit
CCTACGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGATG1220.1775681890955666No Hit
CCTACGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGATG1220.1775681890955666No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1170.17029080429656798No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1120.16301341949756934No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1100.1601024655779699No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1090.15864698861817017No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC890.12953744942217563No Hit
CATACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT860.12517101854277649No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT850.12371554158297675No Hit
CCTACGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGACG800.11643815678397812No Hit
ACTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT770.11207172590457894No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT770.11207172590457894No Hit
CCTACGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGACG710.10333886414578058No Hit
CATACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT710.10333886414578058No Hit
CCTACGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGATG700.10188338718598086No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)