FastQCFastQC Report
Wed 9 Aug 2023
EGAF00008119447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56340
Sequences flagged as poor quality0
Sequence length301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT12802.2719204827831025No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC11932.1175008874689385No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8431.496272630457934No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7941.4093006744763932No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7891.4004259850905219No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7881.3986510472133475No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT7731.372026979055733No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC7601.348952786652467No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7211.2797302094426695No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6941.2318068867589633No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6431.1412850550230742No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6321.1217607383741568No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5701.0117145899893505No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTAGACAATGGGGGCAACCCTGAT5701.0117145899893505No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5610.9957401490947817No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC5540.9833155839545616No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5220.926517571884984No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC4990.8856940007099752No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4740.8413205537806177No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4480.7951721689740858No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4470.7933972310969116No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT4420.7845225417110401No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4420.7845225417110401No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTAGACAATGGGGGCAACCCTGAT4120.7312744053958111No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4060.7206247781327654No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4040.7170749023784168No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3810.6762513312034079No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTAGACAATGGGGGCAACCCTGAT3760.6673766418175363No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3710.6585019524316649No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3580.6354277600283991No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3470.6159034433794817No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTAGACAATGGGGGCAACCCTGAT3380.5999290024849131No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3340.5928292509762159No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTAGACAATGGGGGCAACCCTGAT3320.5892793752218672No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3230.5733049343272986No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3160.5608803691870784No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3130.5555555555555556No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2970.5271565495207668No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT2830.5023074192403266No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTAGACAATGGGGGCAACCCTGAT2800.4969826056088037No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT2710.481008164714235No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT2690.47745828895988635No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2680.47568335108271215No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2670.4739084132055378No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTAGACAATGGGGGCAACCCTGAT2650.47035853745118916No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2560.45438409655662054No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2560.45438409655662054No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2380.42243521476748314No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2360.41888533901313457No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT2320.4117855875044373No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2310.4100106496272631No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2300.40823571175008877No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2290.4064607738729144No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT2270.4029108981185659No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTAGACAATGGGGGCAACCCTGAT2240.3975860844870429No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2180.38693645722399717No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2090.3709620163294285No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2050.36386226482073125No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2020.3585374511892084No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1950.34611288604898827No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1920.3407880724174654No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1910.3390131345402911No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1790.31771388001419953No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1780.31593894213702517No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT1700.3017394391196308No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC1690.29996450124245655No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT1660.2946396876109336No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1650.2928647497337593No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC1640.291089811856585No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1570.27866524671636494No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1550.2751153709620163No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1550.2751153709620163No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGGAACCCTGAT1390.24671636492722754No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1390.24671636492722754No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1380.24494142705005326No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1380.24494142705005326No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1340.23784167554135607No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1330.23606673766418174No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1320.23429179978700748No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1310.23251686190983314No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1270.22541711040113596No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1250.22186723464678737No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC1230.21831735889243875No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1220.2165424210152645No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1220.2165424210152645No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1180.20944266950656729No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC1180.20944266950656729No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1170.20766773162939298No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1110.19701810436634717No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC1100.1952431664891729No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1100.1952431664891729No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1050.1863684771033014No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC1050.1863684771033014No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1040.1845935392261271No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1010.1792687255946042No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC990.1757188498402556No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC980.17394391196308132No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT940.16684416045438408No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGGAACCCTGAT930.1650692225772098No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT910.1615193468228612No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGGAACCCTGAT900.1597444089456869No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC890.15796947106851258No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC870.154419595314164No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT860.1526446574369897No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC850.1508697195598154No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGATGGAAATCTGAA850.1508697195598154No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC830.1473198438054668No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGGAACCCTGAT820.1455449059282925No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC810.14376996805111822No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC810.14376996805111822No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC780.13844515441959532No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT780.13844515441959532No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC780.13844515441959532No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC770.136670216542421No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC760.13489527866524673No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC750.13312034078807242No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC720.12779552715654952No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGGAACCCTGAT700.12424565140220092No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC680.12069577564785233No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT670.11892083777067804No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT660.11714589989350374No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC660.11714589989350374No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT660.11714589989350374No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT640.11359602413915514No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGGAACCCTGAT630.11182108626198084No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC580.10294639687610932No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC580.10294639687610932No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT580.10294639687610932No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT580.10294639687610932No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGATGGAAATCTGAA580.10294639687610932No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC570.10117145899893502No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC570.10117145899893502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)