FastQCFastQC Report
Wed 8 May 2024
EGAF00008119451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184519
Total Bases55.5 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT37582.036646632596101No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC30891.6740823438236712No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC28601.549975883242376No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT25161.3635452175656708No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT20871.1310488350793144No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT20771.1256293389840613No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT20611.1169581452316564No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT20351.1028674553839986No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC20321.1012416065554225No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC19541.0589695370124486No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC18170.9847224405074816No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC17860.9679220026121972No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC17780.9635864057359946No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC17580.9527474135454886No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC16960.9191465377549196No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT16420.889881258840553No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC16190.877416417821471No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC16120.8736227705547939No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT16020.8682032744595407No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC15460.8378540963261236No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT15460.8378540963261236No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC13140.7121217869162525No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC13040.7067022908209994No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT11800.6395005392398615No Hit
CCTACGGGAGGCAGCAGCAACCACCACTAAAATGCTAACGGGGTCCAATG11350.6151128068112227No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT10680.5788021829730271No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT10510.569589039611097No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT10440.5657953923444198No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10210.5533305513253378No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC9450.5121423810014145No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT9430.5110584817823639No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT9260.5018453384204337No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT9200.4985936407632818No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT9180.4975097415442312No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT9160.4964258423251806No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT9120.4942580438870794No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8900.4823351524775227No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8900.4823351524775227No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT8820.4779995556013202No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC8820.4779995556013202No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC8670.46987031145844055No Hit
CCTACGGGAGGCAGCAGGCTTGGACTCTACTTTTTGTCATCCTTCCATCT8440.45740547043935853No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT8260.4476503774679031No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT7960.4313918891821439No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7250.3929134669058471No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT6960.3771969282296132No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6920.375029129791512No Hit
CCTACGGGGGGCAGCAGCAACCACCACTAAAATGCTAACGGGGTCCAATG6840.3706935329153095No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6780.36744183525815766No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6650.36039649033432875No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6380.34576385087714545No Hit
CCTACGGGTGGCTGCAGCAACCACCACTAAAATGCTAACGGGGTCCAATG6290.3408863043914177No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT6220.33709265712474057No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6210.33655070751521526No Hit
CCTACGGGTGGCAGCAGCAACCACCACTAAAATGCTAACGGGGTCCAATG6060.32842146337233563No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC6050.3278795137628103No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5950.3224600176675573No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT5770.3127049246961017No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC5710.3094532270389499No Hit
CCTACGGGGGGCTGCAGCAACCACCACTAAAATGCTAACGGGGTCCAATG5640.30565957977227276No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5610.30403373094369684No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT5560.3013239828960703No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5530.2996981340674944No Hit
CCTACGGGGGGCAGCAGGCTTGGACTCTACTTTTTGTCATCCTTCCATCT5470.2964464364103426No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5420.29373668836271605No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5380.2915688899246148No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5100.2763943008579062No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC5100.2763943008579062No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT5080.2753104016388556No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5080.2753104016388556No Hit
CCTACGGGCGGCTGCAGCAACCACCACTAAAATGCTAACGGGGTCCAATG5010.2715167543721785No Hit
CCTACGGGCGGCAGCAGCAACCACCACTAAAATGCTAACGGGGTCCAATG4990.27043285515312787No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4960.26880700632455196No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4960.26880700632455196No Hit
CCTACGGGCGGCAGCAGGCTTGGACTCTACTTTTTGTCATCCTTCCATCT4850.26284556061977354No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4840.2623036110102483No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4790.2595938629626217No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4750.2574260645245205No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4740.2568841149149952No Hit
CCTACGGGTGGCTGCAGGCTTGGACTCTACTTTTTGTCATCCTTCCATCT4740.2568841149149952No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4740.2568841149149952No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC4690.25417436686736866No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC4680.25363241725784336No Hit
CCTACGGGGGGCTGCAGGCTTGGACTCTACTTTTTGTCATCCTTCCATCT4670.25309046764831805No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4650.25200656842926744No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4620.2503807196006915No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT4550.24658707233401436No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT4510.24441927389591317No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4500.24387732428638786No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4380.23737392897208417No Hit
CCTACGGGTGGCAGCAGGCTTGGACTCTACTTTTTGTCATCCTTCCATCT4250.2303285840482552No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4200.22761883600062865No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4140.22436713834347682No Hit
CCTACGGGCGGCTGCAGGCTTGGACTCTACTTTTTGTCATCCTTCCATCT4040.21894764224822377No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT3930.2129861965434454No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC3920.2124442469339201No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3730.20214720435293926No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT3680.19943745630531273No Hit
CCTACGGGAGGCAGCAGTGATAAATATTAAGCAATGAACGAAAGTTTGAC3640.19726965786721148No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3430.18588871606718008No Hit
CCTACGGGAGGCTGCAGCAACCACCACTAAAATGCTAACGGGGTCCAATG3280.17775947192430047No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT3210.17396582465762334No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3160.1712560766099968No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3120.16908827817189556No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT2930.15879123559091476No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2910.15770733637186413No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT2820.15282978988613638No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2750.14903614261945924No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2680.1452424953527821No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT2620.14199079769563025No Hit
CCTACGGGAGGCTGCAGGCTTGGACTCTACTTTTTGTCATCCTTCCATCT2560.13873910003847842No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT2510.1360293519908519No Hit
CCTACGGGGGGCAGCAGTGATAAATATTAAGCAATGAACGAAAGTTTGAC2490.13494545277180128No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2450.13277765433370006No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT2430.13169375511464942No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2420.13115180550512415No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2350.12735815823844698No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT2340.12681620862892168No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT2330.12627425901939637No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT2320.12573230940987107No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC2320.12573230940987107No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2280.12356451097176985No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT2240.12139671253366861No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC2240.12139671253366861No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT2180.11814501487651678No Hit
CCTACGGGGGGCTGCAGTGATAAATATTAAGCAATGAACGAAAGTTTGAC2180.11814501487651678No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT2160.11706111565746617No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2150.11651916604794087No Hit
CCTACGGGTGGCTGCAGTGATAAATATTAAGCAATGAACGAAAGTTTGAC2140.11597721643841555No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2130.11543526682889024No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT2110.11435136760983963No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1990.10784797229553597No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT1980.10730602268601065No Hit
CCTACGGGTGGCAGCAGTGATAAATATTAAGCAATGAACGAAAGTTTGAC1980.10730602268601065No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1940.10513822424790943No Hit
CCTACGGGCGGCAGCAGTGATAAATATTAAGCAATGAACGAAAGTTTGAC1910.10351237541933352No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT1900.1029704258098082No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1860.10080262737170698No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)