FastQCFastQC Report
Wed 8 May 2024
EGAF00008119461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34994
Total Bases10.5 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6921.9774818540321197No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4381.2516431388237983No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4321.2344973424015546No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4301.228782076927473No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4231.2087786477681888No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3781.0801851746013602No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3681.051608847230954No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3180.9087272103789221No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3150.9001543121678002No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2690.768703206263931No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2660.760130308052809No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2590.7401268788935246No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2530.7229810824712808No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2140.611533405726696No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2130.6086757729896554No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2130.6086757729896554No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1970.5629536491970052No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1930.5515231182488427No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1930.5515231182488427No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1840.525804423615477No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1790.5115162599302738No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1480.422929645082014No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1400.400068583185689No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1310.37434988855232326No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1290.36863462307824196No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1210.34577356118191693No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1150.3286277647596731No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1100.3143396010744699No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1060.30290907012630736No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT980.28004800822998227No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT970.2771903754929416No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT960.274332742755901No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT920.2629022118077385No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT860.24575641538549467No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT840.2400411499114134No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT820.23432588443733213No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT810.23146825170029145No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT790.22575298622621018No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT770.22003772075212896No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT770.22003772075212896No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT770.22003772075212896No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT760.21718008801508829No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT700.2000342915928445No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT690.19717665885580385No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT670.19146139338172258No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT650.1857461279076413No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT650.1857461279076413No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT630.18003086243356003No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT630.18003086243356003No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT630.18003086243356003No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT620.1771732296965194No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT620.1771732296965194No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT590.1686003314853975No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT580.16574269874835687No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT570.16288506601131622No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT570.16288506601131622No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT560.1600274332742756No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT560.1600274332742756No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT550.15716980053723495No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT550.15716980053723495No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT540.15431216780019433No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT540.15431216780019433No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT540.15431216780019433No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT530.15145453506315368No Hit
CCTACGGGAGGCAGCAGGATTAGATACCCTTGTAGTCCTGTCTCTTATAC530.15145453506315368No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT530.15145453506315368No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT530.15145453506315368No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT520.14859690232611303No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT520.14859690232611303No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT520.14859690232611303No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT510.14573926958907243No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT510.14573926958907243No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT500.14288163685203178No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT500.14288163685203178No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT500.14288163685203178No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT490.14002400411499114No Hit
CCTACGGGAGGCAGCAGGATTAGATACCCTAGTAGTCCTGTCTCTTATAC490.14002400411499114No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT490.14002400411499114No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT490.14002400411499114No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT480.1371663713779505No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT480.1371663713779505No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT480.1371663713779505No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT470.13430873864090986No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT460.13145110590386924No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT460.13145110590386924No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT460.13145110590386924No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCCAGTAGTCCTGTCTCT460.13145110590386924No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCGGGTAGTCCTGTCTCT460.13145110590386924No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT450.1285934731668286No Hit
CCTACGGGAGGCAGCAGGATTAGATACCCCGGTAGTCCTGTCTCTTATAC450.1285934731668286No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT440.12573584042978794No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT440.12573584042978794No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT440.12573584042978794No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT440.12573584042978794No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT430.12287820769274733No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT430.12287820769274733No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT430.12287820769274733No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT430.12287820769274733No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT430.12287820769274733No Hit
CCTACGGGAGGCAGCAGGATTAGATACCCCTGTAGTCCTGTCTCTTATAC420.1200205749557067No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT420.1200205749557067No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT420.1200205749557067No Hit
CCTACGGGAGGCAGCAGGATTAGATACCCGAGTAGTCCTGTCTCTTATAC410.11716294221866606No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC410.11716294221866606No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT410.11716294221866606No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT410.11716294221866606No Hit
CCTACGGGAGGCAGCAGGATTAGATACCCTGGTAGTCCTGTCTCTTATAC400.11430530948162543No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT400.11430530948162543No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCCTGTAGTCCTGTCTCT390.11144767674458478No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC390.11144767674458478No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT390.11144767674458478No Hit
CCTACGGGAGGCAGCAGGATTAGATACCCCAGTAGTCCTGTCTCTTATAC390.11144767674458478No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCGAGTAGTCCTGTCTCT390.11144767674458478No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT390.11144767674458478No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT380.10859004400754414No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT380.10859004400754414No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT380.10859004400754414No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT380.10859004400754414No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCCGGTAGTCCTGTCTCT380.10859004400754414No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT370.1057324112705035No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCGTGTAGTCCTGTCTCT370.1057324112705035No Hit
CCTACGGGAGGCAGCAGTGGCGAAGGCGGATTGCTGGACAGTAACTGACG370.1057324112705035No Hit
CCTACGGGAGGCAGCAGGATTAGATACCCGTGTAGTCCTGTCTCTTATAC360.10287477853346287No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCTTGTAGTCCTGTCTCT360.10287477853346287No Hit
CCTACGGGAGGCTGCAGATGTGGATTAGATACCCGGGTAGTCCTGTCTCT350.10001714579642225No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT350.10001714579642225No Hit
CCTACGGGGGGCTGCAGATGTGGATTAGATACCCTTGTAGTCCTGTCTCT350.10001714579642225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)