FastQCFastQC Report
Wed 8 May 2024
EGAF00008119462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34994
Total Bases10.5 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5701.628850660113162No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5241.497399554209293No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4951.4145282048351147No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4541.2973652626164485No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4391.2545007715608392No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4181.1944904840829857No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4081.1659141567125795No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4081.1659141567125795No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3941.1259072983940104No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2510.7172658169971995No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2240.6401097330971024No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2070.5915299765674116No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1940.5543807509858832No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1830.5229467908784363No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1740.4972280962450706No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1680.4800822998228268No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1600.4572212379265017No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1530.4372178087672173No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1530.4372178087672173No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1490.4257872778190547No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1460.4172143796079328No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1420.40578384865977024No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1390.39721095044864835No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1350.3857804195004858No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1290.36863462307824196No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1190.34005829570783563No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1160.33148539749671374No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT1090.3114819683374293No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT1050.30005143738926676No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT980.28004800822998227No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT930.26575984454477913No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG890.2543293135966166No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT860.24575641538549467No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG810.23146825170029145No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT810.23146825170029145No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG780.22289535348916956No Hit
GACTACACGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT740.211464822541007No Hit
GACTACAGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT730.2086071898039664No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG720.20574955706692574No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG700.2000342915928445No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA690.19717665885580385No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT680.1943190261187632No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG650.1857461279076413No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG650.1857461279076413No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG640.18288849517060068No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT620.1771732296965194No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG620.1771732296965194No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA610.17431559695947876No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT610.17431559695947876No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT600.17145796422243811No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT600.17145796422243811No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT590.1686003314853975No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG580.16574269874835687No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG570.16288506601131622No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG560.1600274332742756No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT560.1600274332742756No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA550.15716980053723495No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG550.15716980053723495No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT540.15431216780019433No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT530.15145453506315368No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG520.14859690232611303No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCAGCCTCCCGTAGGCTGTCTCT520.14859690232611303No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT510.14573926958907243No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA510.14573926958907243No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT510.14573926958907243No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA500.14288163685203178No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT500.14288163685203178No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT490.14002400411499114No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT490.14002400411499114No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA490.14002400411499114No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG490.14002400411499114No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG490.14002400411499114No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT490.14002400411499114No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG490.14002400411499114No Hit
GACTACTAGGGTATCTAATCCTGCTGCCTCCCGTAGGCTGTCTCTTATAC490.14002400411499114No Hit
GACTACTGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT470.13430873864090986No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT470.13430873864090986No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT470.13430873864090986No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT470.13430873864090986No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG470.13430873864090986No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT460.13145110590386924No Hit
GACTACAAGGGTATCTAATCCTGCTGCCTCCCGTAGGCTGTCTCTTATAC460.13145110590386924No Hit
GACTACACGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT460.13145110590386924No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG460.13145110590386924No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT460.13145110590386924No Hit
GACTACCCGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT460.13145110590386924No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT460.13145110590386924No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT460.13145110590386924No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCTCCCGTAGGCTGTCTCT460.13145110590386924No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG450.1285934731668286No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT450.1285934731668286No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG440.12573584042978794No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCTCCCGTAGGCTGTCTCT440.12573584042978794No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA440.12573584042978794No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT440.12573584042978794No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT440.12573584042978794No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT440.12573584042978794No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT440.12573584042978794No Hit
GACTACAGGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT430.12287820769274733No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT420.1200205749557067No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT420.1200205749557067No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCAGCCTCCCGTAGGCTGTCTCT420.1200205749557067No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG420.1200205749557067No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT420.1200205749557067No Hit
GACTACACGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT420.1200205749557067No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT420.1200205749557067No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCAGCCTCCCGTAGGCTGTCTCT410.11716294221866606No Hit
GACTACCAGGGTATCTAATCCTGCTGCCTCCCGTAGGCTGTCTCTTATAC410.11716294221866606No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG410.11716294221866606No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG410.11716294221866606No Hit
GACTACCGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT400.11430530948162543No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC400.11430530948162543No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCTCCCGTAGGCTGTCTCT400.11430530948162543No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC390.11144767674458478No Hit
GACTACTCGGGTATCTAATCCTGCTGCCTCCCGTAGGCTGTCTCTTATAC380.10859004400754414No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT380.10859004400754414No Hit
GACTACAGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT380.10859004400754414No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT380.10859004400754414No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA380.10859004400754414No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT380.10859004400754414No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC380.10859004400754414No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT380.10859004400754414No Hit
GACTACCGGGGTATCTAATCCTGCTGCCTCCCGTAGGCTGTCTCTTATAC380.10859004400754414No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA370.1057324112705035No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG370.1057324112705035No Hit
GACTACACGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT370.1057324112705035No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT370.1057324112705035No Hit
GACTACAAGGGTATCTAATCCACATCTGCAGCCCCCCGTAGGCTGTCTCT370.1057324112705035No Hit
GACTACAGGGGTATCTAATCCTGCTGCCTCCCGTAGGCTGTCTCTTATAC370.1057324112705035No Hit
GACTACTGGGGTATCTAATCCTGCTGCCTCCCGTAGGCTGTCTCTTATAC370.1057324112705035No Hit
GACTACAGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT370.1057324112705035No Hit
GACTACACGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT360.10287477853346287No Hit
GACTACACGGGTATCTAATCCTGCTGCCTCCCGTAGGCTGTCTCTTATAC360.10287477853346287No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT360.10287477853346287No Hit
GACTACTCGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT360.10287477853346287No Hit
GACTACACGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT350.10001714579642225No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT350.10001714579642225No Hit
GACTACCAGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT350.10001714579642225No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC350.10001714579642225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)